P16功能性短肽对C6和U251细胞增殖的抑制作用

J4 ›› 2011, Vol. 37 ›› Issue (2): 264-266.

P16功能性短肽对C6和U251细胞增殖的抑制作用

孙立超¹|李蕴潜²|宋红梅¹|朱战鹏¹|徐保锋²|马程远²|邱吉庆¹

1.吉林大学第一医院神经内科癫痫治疗中心|吉林长春130021；2.吉林大学第一医院神经外科,吉林长春130021

收稿日期:2010-08-25 出版日期:2011-03-28 发布日期:2011-03-28
通讯作者: 邱吉庆(Tel:0431-88782236,E-mail:qiujiqing2008@163.com) E-mail:qiujiqing2008@163.com
作者简介:孙立超（1984-）|男,黑龙江省牡丹江市人|医师|医学硕士|主要从事立体定向功能神经外科方面的研究。
基金资助:
吉林省科技厅科研基金资助课题（200705153）；长春市科技局社会发展项目资助课题（2008089）

Inhibitory effects of P16 functional oligopeptide on proliferation of C6 cells and U251 cells

SUN Li-chao¹,LI Yun-qian²,SONG Hong-mei¹,ZHU Zhan-peng¹|XU Bao-feng²,MA Cheng-yuan²,Qiu Ji-qing¹

1.Epilepsy Therapy Center,Department of Neurology|First Hospital,Jilin University,Changchun 130021,China；〖JP〗2.Department of Neurosurgery|First Hospital,Jilin University,Changchun 130021,China

Received:2010-08-25 Online:2011-03-28 Published:2011-03-28

摘要/Abstract

摘要：

目的：探讨P16功能性短肽对体外培养的小鼠脑胶质瘤C6细胞和人神经胶质瘤U251细胞增殖的抑制作用。方法：以不同剂量的P16功能性短肽(12.5、25.0、50.0和100.0 mg·L^-1）分别作用于小鼠脑胶质瘤C6细胞和人神经胶质瘤U251细胞24、48和72 h，同时设不加药的阴性对照组，应用MTT比色法检测细胞增殖抑制率。结果：12.5、25.0、50.0和100.0 mg·L^-1的P16功能性短肽均可抑制C6和U251细胞的增殖，其细胞增殖抑制率高于阴性对照组(P<0.01)；随着剂量的增加及作用时间的延长，C6和U251细胞增殖抑制率增加。结论：P16功能性短肽能抑制小鼠脑胶质瘤C6细胞和人神经胶质瘤U251细胞的生长。

关键词: P16功能性短肽；胶质瘤；C6细胞；U251细胞；细胞增殖

Abstract:

Abstract:Objective To explore the inhibitory effects of P16 functional oligopeptide on proliferation of mouse C6 glioma cells and human U251 glioma cells cul
tivated in vitro. Methods The C6 cells and U251 cells were treated with different concentrations of P16 functional oligopeptide (12.5,25.0,50.0 and 100.0 mg·L^-1) for 24,48 and 72 h,the negative control group(without P16 functional oligopeptide) was set up at the same time.The inhibitory rates of proliferation of C6 cells and U251 cells were detected with MTT. Results 12.5,25.0,50.0 and 100.0 mg·L^-1 P16 functional oligopeptide inhibited the proliferation of C6 cells and U251 cells. The inhibitory rates of different doses of P16 functional oligopeptide were higher than that in negative control group(P＜0.01).The inhibitory rate was increased with the increasing of dose andprolongation of time．Conclusion P16 functional oligopeptide can inhibit the growth of C6 cells and U251 cells.

Key words: P16 functional oligopeptide；glioma；C6 cells；U251 cells;cell proliferation

中图分类号:

R730.264

孙立超|李蕴潜|宋红梅|朱战鹏|徐保锋|马程远|邱吉庆. P16功能性短肽对C6和U251细胞增殖的抑制作用[J]. J4, 2011, 37(2): 264-266.