关键词: 移植, 烧伤, 细胞移植

Abstract: Objective To observe the curative effects of deep partial thickness derma burn injury using bone marrow mesenchymal stem cells. Methods The bone marrow stem cells were obtained from the male guinea pig and cultured in vitro. Then the mesenchymal stem cells (MSCs) with 2×10⁶ cell•mL^-1 and 2×10⁷ cell•mL^-1 were prepared, and transplanted into the wounds of the female guinea pig models with deep partial thickness derma burn injury. The wound healing area was counted and the morphological changes were observed by using HE and Masson stain. Results The wound healing in therapy groups were significantly better than that in control groups. HE stain showed that dermal and epidermal structures were clear, and cell structures of every epidermal layers were distincted and integrated in therapy groups,but cell structures of every layers in control groups were unclear. Masson stain showed that collagen was regular and new-born epidermal covered the wound in therapy groups, collagen was disorder and thicker in control groups. Bone marrow MSCs could promote the healing of wound and the effects in therapy groups were better than that in the control groups. But there was no significantly difference between MSCs with 2×10⁶ cell•mL^-1 and 2×10⁷ cell•mL^-1 in therapy groups. Conclusion The bone marrow MSCs have significant curative effects on deep partial thickness derma burn injury.

Key words: transplantation, burns, cell transplantation