关键词: 基因疗法, 放射治疗, 神经胶质瘤, 细胞凋亡

Abstract: Objective To investigate the effect of pEgr-hPTEN stable transfer combined with irradiation on the proliferation and apoptosis of SHG-44 human glioma cells in vitro. Methods pEgr-hPTEN vector containing the exogenous wild type PTEN gene was transfected into SHG-44 cells under mediation of lipofectamine in vitro, positive cell clones were selected and amplified. Western blotting was used to detect the properties of PTEN expression induced by X-ray irradiation. Flow cytometry and cell growth curve were adopted to measure the effects of PTEN gene transfer combined with different doses of X-ray irradiation on cell proliferation and apoptosis of the transfected SHG-44 cells. Results Expression of PTEN protein could be enhanced by X-ray irradiation in SHG-44-hPTEN stable transfer cells. PTEN protein relative level was in dose-dependent manner within 5 Gy. pEgr-hPTEN stable transfer combined with X-ray irradiation could significantly inhibit the proliferation and induce apoptosis of SHG-44 cells. At the 8th day after irradiation with different doses of X-ray, the numbers of SHG-44-hPTEN stable transfer cells were only 30.0%-50.0% of that of SHG-44-hPTEN/0 Gy group and 7.7%-13.0% of SHG-44/0 Gy group. The percentage of early apoptotic cells of SHG-44-hPTEN group after irradiation with X-ray irradiated were 1.5-2.3 times as much as that of SHG-44-hPTEN/0 Gy group, 1.9-4.4 times as much as that of SHG-44 irradiated group and 3.4-5.1 times as much as that of SHG-44 /0 Gy group. Conclusion The apoptosis of tumor cells could be significantly enhanced and its growth could be significantly inhibited by gene -radiotherapy in vitro.

Key words: gene therapy, radiotherapy, glioma, apoptosis