J4 ›› 2010, Vol. 36 ›› Issue (2): 281-284.

• 基础研究 • 上一篇    下一篇

下丘脑视前区性二态核神经元的投射

张春晓1, 滨田知宏2, 黄民1   

  1. 1.吉林大学基础医学院生理学系|吉林 长春 130021;2.日本医科大学生理学系|日本 东京 1138602
  • 收稿日期:2009-09-06 出版日期:2010-03-28 发布日期:2010-03-28
  • 通讯作者: 张春晓(Tel:0431-85619472, E-mail:zcxmb@yahoo.com.cn) E-mail:zcxmb@yahoo.com.cn
  • 作者简介:张春晓(1968-)|女|辽宁省丹东市人|副教授|医学博士|主要从事神经生理学研究。
  • 基金资助:

    卫生部笹川医学奖学金项目资助课题(2007);吉林省卫生厅科研基金资助课题(2008Z069)

Nerve connections of sexually dimorphic nucleus of preoptic area

 ZHANG Chun-Xiao1, BIN Tian-Zhi-Hong2, HUANG Min1   

  1. 1. Department of Physiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China;2. Department of Physiology,Nippon Medical School, Tokyo 1138602, Japan
  • Received:2009-09-06 Online:2010-03-28 Published:2010-03-28

摘要:

目的:检查大鼠下丘脑视前区性二态核(SDN-POA)的神经投射部位,探讨SDN-POA的功能。方法:实验使用30只Wistar转基因大鼠,这种转基因大鼠的雌激素α受体基因启动子0/B上标记有绿色荧光蛋白。雌、雄性大鼠(各15只)随机各分成3组,分别通过离子电渗法将示踪剂Fluoro-Ruby注入下丘脑腹内侧核(VMH)、终纹床核(BST)、杏仁核后背侧核(MePD)。5 d后,灌流固定鼠脑,做冷冻冠状切片,荧光显微镜下确定示踪剂注入位置,并观察SDN-POA绿色荧光和Fluoro-Ruby标记荧光。结果:在雌、雄性转基因大鼠,SDN-POA呈现绿色荧光,雄性SDN的体积和细胞数量明显大于雌性,即存在明显的两性差异。示踪剂注入VMN和BST组,在SDN均发现Fluoro-Ruby逆行标记细胞。来自VMH的标记细胞主要位于SDN的边缘;来自BST的标记细胞分散在SDN核团内。示踪剂注入MePD组,在SDN未发现逆行标记的神经元,而发现大量标记Fluoro-Ruby的神经纤维。结论:SDN-POA是决定性二态性的重要中枢部位,它可接受MePD的传入神经纤维,发出传出神经纤维影响VMN和BST神经元的活动,进而调节生殖功能。

关键词: 视前区性二态核;性差异;逆行示踪研究;Fluoro-Ruby

Abstract:

Abstract:Objective To examine the nerve connections of the sexually dimorphic nucleus of the preoptic area (SDN-POA) in the rat, and explore the function of  SDN-POA.Methods A total of 30 transgenic rats were used in the study,which expressed enhanced green fluorescent protein (EGFP) under the control of an estrogen receptor α gene promoter 0/B.The male and female rats (n=15, respectively) were randomly divided into three groups, and Fluoro-Ruby, a fluorescent tracer, was stereotaxically injected into the ventromedial hypothalamus (VMH), bed nucleus of the stria terminalis (BST) and posterodorsal nucleus of the amygdale (MePD) by iontophoresis, respectively.Five days later, the rats were perfused, and the sections containing SDN-POA region and site of injection were cut on a freezing microtome.the sections were viewed to assess extent of tracer deposition in the injection site and to reveal the EGFP or Fluoro-Ruby fluorescence in SDN-POA under fluorescence microscope.Results In male and female transgenic rats,  the cells labeled with EGFP were specifically distributed in the SDN-POA, and the volume and the number of cells in SDN were larger in the male than female and it demonstrated the sex difference.In the groups injected with tracer into the VMH and BST, the cells labeled with Fluoro-Ruby were determined in SDN.The labeled cells from VMH were distributed in the later of the SDN and the labeled cells from BST were dispersedly distributed in SDN in both sexes.In the group injected with tracer into the MePD, the labeled nerve fibers,not neurons,were discovered in SDN in both sexes.Conclusion SDN-POA is an important center nucleus to determine sexual dimorphism, and it would receive the afferent nerve fiber from MePD and send the efferent nerve fiber to VMN and BST to regulate reproductio
n.

Key words: sexually dimorphic nucleus of the preoptic area;sex differences;retrograde tracing study;Fluoro-Ruby

中图分类号: 

  • R-332