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蒺藜皂苷对大鼠在体结扎冠脉造成的急性心肌缺血损伤的保护作用

魏征人，李 红，杨世杰*   

  1. 吉林大学基础医学院药理学教研室，吉林 长春130021
  • 收稿日期:2004-09-29 修回日期:1900-01-01 出版日期:2005-01-28 发布日期:2005-01-28
  • 通讯作者: 杨世杰

Protective effects of Gross Saponins of Tribulus Terrestris on acute myocardial ischemia induced by ligatingleft coronary artery in rats in vivo

WEI Zheng-ren,LI Hong,YANG Shi-jie*   

  1. Department of Pharmacology, School of Basic Medical Sciences， Jilin University, Changchun 130021, China
  • Received:2004-09-29 Revised:1900-01-01 Online:2005-01-28 Published:2005-01-28
  • Contact: YANG Shi-jie

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摘要： 目的：研究蒺藜总皂苷（GSTT）对大鼠急性心肌缺血损伤的保护作用。 方法：结扎大鼠左冠状动脉前降支建立急性心肌缺血模型，观察GSTT对心肌缺血大鼠血清LDH、SOD和MDA的影响；通过HE染色、电镜观察心肌细胞形态学的变化；采用TUNEL方法半定量观察GSTT对凋亡细胞阳性率的影响。 结果：与对照组比较，GSTT可降低心肌缺血大鼠血清LDH、MDA（P<0.01），升高SOD(P<0.01)，降低心肌凋亡细胞阳性率(P<0.01)；模型组大鼠心肌细胞结构出现明显的病理改变。 结论：GSTT对结扎大鼠左冠状动脉前降支引起的急性心肌缺血损伤具有保护作用，保护作用通过升高SOD，清除自由基的能力增强及LDH的释出减少进而抑制心肌细胞凋亡。

关键词: 药理学, 疾病模型, 动物, 心肌缺血, 细胞凋亡, 药物作用, 蒺藜皂苷

Abstract: Objective To study the protective effects of Gross Saponins of Tribulus Terrestris (GSTT) on acute myocardial ischemia in rats. Methods An acute myocardial ischemic model was set up by ligating left coronary artery, the effects of GSTT on the serum LDH, SOD, and MDA contents in rats were observed. Meanwhile, HE staining and electron microscope observation were used to study the morphological changes of cardiomyocytes. The TUNEL semi-quantitative technique was performed to observe the effects of GSTT on apoptosis rate. Results Compared with control group, GSTT decreased the serum LDH and MDA contents（P<0.01）,while it increased the serum SOD level (P<0.01) in rats. It decreased myocardial apoptosis rate as well(P<0.01). The obvious pathological changes of cardiomyocyte structure were found in model group. Conclusion GSTT has protective effects on acute myocardial ischemia induced by ligating left coronary artery in rats in vivo, which is closely related to enhancing removement of free radicals by increasing SOD content, decreasing the leakage of LDH, and inhibiting the apoptosis of cardiomyocytes.

Key words: pharmacology, disease model, animal, myocardial ishemia, apoptosis, drug effects, Gross Saponins of Tribulus Terrestris

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  • R972
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