假丝酵母性阴道炎的病原菌检测及RAPD分析

J4 ›› 2010, Vol. 36 ›› Issue (3): 527-530.

假丝酵母性阴道炎的病原菌检测及RAPD分析

韩淑梅^1,2, 贺丹¹, 张晓霞², 张宇¹, 许丽波¹, 田庄¹, 横山耕治³, 王丽¹

（1.吉林大学基础医学院病原生物学教研室,吉林长春 130021;2. 吉林大学第一医院妇产科,吉林长春 130021;3.日本千叶大学真菌医学研究中心,日本千叶 260-8673）

收稿日期:2009-11-27 出版日期:2010-05-28 发布日期:2010-05-28
通讯作者: 王丽 E-mail:wli99@jlu.edu.cn
作者简介:韩淑梅（1963－）|女|吉林省长春市人|主管技师|主要从事妇科病原学检查及妇科细胞病理的相关研究。
基金资助:
国家自然科学基金资助课题（30571773）；吉林省科技厅科技发展计划重点项目资助课题（20080444-2）

Pathogen detection and RAPD analysis of candidal vaginitis

HAN Chu-Mei^1,2, HE Dan¹, ZHANG Xiao-Xia², ZHANG Yu¹, HU Li-Bo¹, TIAN Peng¹, GUANG Shan-Geng-Chi³, WANG Li¹

(1. Department of Pathogenobiology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China;2. Department of Obstetrics and Gynecology,First Hospital,Jilin University,Changchun 130021,China;3. Medical Mycology Research Center,Chiba University,Chiba 260-8673,Japan)

Received:2009-11-27 Online:2010-05-28 Published:2010-05-28

摘要/Abstract

摘要：

目的：探讨女性假丝酵母性阴道炎的病原菌种类，分析其随机扩增DNA多态性（RAPD），为该病的临床诊断及流行病学监测提供依据。方法：刮取106例疑似假丝酵母性阴道炎患者道分泌物，分离培养并鉴定病原菌；利用10种随机引物（RAPD 1~10）对分离病原菌进行RAPD分析。结果：①分离获得假丝酵母72株，其中白假丝酵母56株（77.78%）,非白假丝酵母16株（22.22%），包括光滑假丝酵母6 株（8.33%）、热带假丝酵母4 株（5.56%）、克柔假丝酵母1 株（1.39%）及其他假丝酵母5 株（6.94%）；②RAPD分析表明：有2种引物（RAPD2和RAPD5）可以获得较清晰、稳定的特异性带型，具有较明显的种间遗传变异性和种内遗传相似性。结论：假丝酵母性阴道炎病原菌以白假丝酵母为主。随机引物RAPD2和RAPD5比较适于白假丝酵母和热带假丝酵母的分型、鉴定。

关键词: 假丝酵母；假丝酵母性阴道炎；随机扩增DNA多态性

Abstract:

Abstract:Objective
To approach the spectrum of pathogen of candidal vaginitis and analyze the randomly amplified polymorphism DNA (RAPD) of isolated pathogen,and provide basis for clinical diagnosis and epidemiological surveillance of candidal vaginitis.Methods The specimens of vaginal discharge of 106 patients suspected with candidal vaginitis were collected.Pathogenic fungal were isolated and identified.10 random primers (RAPD 1-10) were used to make RAPD analysis on the isolates.Results ①72 strains of Candida were isolated,among which C.albicans was 56 strains (77.78%),non-Candida albicans Candida (NCAC) species was 16 strains (22.22%) including 6 strains of C. glabrata (8.33%),4 strains of C. tropicalis (5.56%),1 strains of C. krusei (1.39%) and 5 strains of other Candida sp. (6.94%).②RAPD analysis result showed that 2 primers (RAPD2 and RAPD5) could have better clear and stabile specific pattern bandings with evident interspecies genetic variability and intraspecies genetic resemblance.Conclusion C.albicans is the main pathogen of candidal vaginitis.RAPD2 and RAPD5 are suitable for identification and typing of C.albicans and C.tropicalis.

Key words: Candida;candidal vaginitis;randomly amplified polymorphism DNA

中图分类号:

R379

韩淑梅, 贺丹, 张晓霞, 张宇, 许丽波, 田庄, 横山耕治, 王丽. 假丝酵母性阴道炎的病原菌检测及RAPD分析[J]. J4, 2010, 36(3): 527-530.

HAN Chu-Mei, HE Dan, ZHANG Xiao-Xia, ZHANG Yu, HU Li-Bei, TIAN Peng, GUANG Shan-Geng-Chi, WANG Li. Pathogen detection and RAPD analysis of candidal vaginitis[J]. J4, 2010, 36(3): 527-530.