HBx和VEGF与乙肝相关性肝癌组织血管生成及转移的关系

J4 ›› 2010, Vol. 36 ›› Issue (2): 372-376.

HBx和VEGF与乙肝相关性肝癌组织血管生成及转移的关系

刘凯歌,高红艳,赵慧,牛春燕,李莉

西安医学院附属医院消化科,陕西西安 710077

收稿日期:2009-09-18 出版日期:2010-03-28 发布日期:2010-03-28
通讯作者: 刘凯歌（Tel:029-84277552,E-mail:kaigeliu@gmail.com） E-mail:kaigeliu@gmail.com
作者简介:刘凯歌（1969-）|男|陕西省礼泉县人|副主任医师|医学硕士|主要从事肝癌的基础及临床研究。
基金资助:
陕西省科技厅自然科学基金资助课题（2007C220）

Correlation between HBx and VEGF expressions and angiogenesis and metastasis in HBV related hepatocellular carcinoma |tissue

LIU Kai-Ge, GAO Hong-Yan, ZHAO Hui, NIU Chun-Yan, LI Li

Department of Digestive Diseases| |Affiliated Hospital, Xi’an Medical College,Xi’an 710077,China

Received:2009-09-18 Online:2010-03-28 Published:2010-03-28

摘要/Abstract

摘要：

目的：探讨乙肝病毒X蛋白(HBx)、血管内皮细胞生长因子(VEGF)在乙肝相关性肝癌组织血管生成及转移过程中的作用。方法：选择84例乙肝相关性肝癌组织和22例非乙肝相关性肝癌组织，免疫组织化学法检测HBx、VEGF及血管内皮细胞表面抗原(CD34)表达，光镜下微血管计数(MVD)。结果:84例乙肝相关性肝癌组织中HBx阳性表达率为73.81%(62／84)，阴性率为26.19% (22／84)；62例HBx阳性表达组中VEGF阳性率［74.19%(46／62)］明显高于22例HBx阴性组［36.36%(8／22)］和非乙肝相关性肝癌组［36.36%(8／22) （P<0.05）］；HBx阴性表达的乙肝相关性肝癌组和非乙肝相关性肝癌组中VEGF阳性表达比较差异无显著性（P>0.05）；84例乙肝相关性肝癌组织中HBx和VEGF的表达水平呈正相关关系（Rs= 0.552,P<0.01）；二者在高分化肿瘤组织中的表达高于低分化组织（P<0.05），有转移组表达高于无转移组； HBx阳性表达组平均MVD值明显高于HBx阴性组和非乙肝相关性肝癌组（P<0.01），有转移组MVD高于无转移组（P<0.01）；有门脉侵犯组高于非侵犯组（P<0.05）。结论：HBx和VEGF广泛表达于乙肝相关性肝癌组织中，二者表达水平呈正相关关系；HBx可能通过上调VEGF的表达在乙肝相关性肝癌组织血管生成及转移中起促进作用。

关键词: 肝肿瘤；乙肝病毒X蛋白；血管内皮生长因子；微血管密度

Abstract:

Abstract:Objective To investigate the corrrelation between hepatitis B virus X protein(HBx) and vascular endothelial growth factor (VEGF) expressions and angiogenesis and metastasis in HBV related hepatocellular carcinoma tissue.Methods Immunohistochemistry was used to detect the expressions of HBx,VEGF and CD34 in 84 cases of HBV related hepatocellular carcinoma and 22 case of non-HBV related hepatocellular carcinoma tissues,the microvascular density(MVD) was recorded under optical microscope.Results The positive expression rate of HBx in 84 cases of HBV related hepatocellular carcinoma tissues was 73.81% (62/84),the negative rate was 26.19% (22／84).The positive expression rate of VEGF in 62 cases of HBx positive expression was 74.19 % (46／62),which was significantly higher than those in 22 hepatocellular carcinoma tissues with HBx negative expression (36.36%,8／22) and 22 non-HBV related hepatocellular carcinoma tissues(36.36%，8/22),while there was no statistically significant difference of VEGF prositive expression between HBV related hepatocellular carcinoma tissue with HBx negative expression and non-HBV related hepaocellular carcinoma tissue(P>0.05).HBx has positive correlation with VEGF（Rs= 0.552,P<0.05).The expressions of HBx and VEGF in well differentiation group were higher than those in poor differentiation group(P<0.05),and the expressions of HBx and VEGF in metastasis group were higher than those in non-metastasis group.The MVD in HBx positive expression group was significantly higher than those in HBx negative expression group and non-HBV related hepacellular carcinoma group (P<0.01),the MVD in metastasis group was higher than that in non-metastasis group (P<0.01),and the MVD in portal vein invasion group was higher than that in non-invasion group (P<0.05).Conclusion HBx and VEGF widely express in HBV related hepatocellular carcinoma tissues,and they have positive relationship.HBx may play an important role in angiogenesis and metastasis in hepatocellular carcinoma by up-regulating the VEGF expression.

Key words: liver neoplasms;hepatitis B virus X protein;vascular endothelial growth factor;microvascular density

中图分类号:

R512.6

刘凯歌, 高红艳, 赵慧, 牛春燕, 李莉. HBx和VEGF与乙肝相关性肝癌组织血管生成及转移的关系[J]. J4, 2010, 36(2): 372-376.

LIU Kai-Ge, GAO Hong-Yan, ZHAO Hui, NIU Chun-Yan, LI Li. Correlation between HBx and VEGF expressions and angiogenesis and metastasis in HBV related hepatocellular carcinoma |tissue[J]. J4, 2010, 36(2): 372-376.