肾细胞癌组织中Notch信号途径蛋白表达水平的检测及其意义

J4 ›› 2011, Vol. 37 ›› Issue (1): 93-96.

肾细胞癌组织中Notch信号途径蛋白表达水平的检测及其意义

娄冬梅^1,2, 牟荣³, 武慧*, 刘玉和³, 钟加滕¹, 于春艳³, 杨冬艳⁴

1.吉林大学白求恩医学院病理生理学教研室|吉林长春 130021；2.吉林省长春市中心医院心血管内科|吉林长春 130051；3. 北华大学基础医学院病理学教研室|吉林吉林 132000； 4.吉林大学中日联谊医院电诊科,吉林长春130033

收稿日期:2010-08-25 出版日期:2011-01-28 发布日期:2011-01-28
通讯作者: 杨冬艳（Tel:0431-84995104，E-mail:yangdongyan2850@163.com） E-mail:yangdongyan2850@163.com
作者简介:娄冬梅(1975-),女,吉林省长春市人|副主任医师|在读医学博士|主要从事肿瘤的病理生理学研究。
基金资助:
吉林省科技厅科技发展计划项目资助课题（200705316）

Determination of expression of Notch signaling pathway protein |in renal cell carcinoma tissues and its significance

LOU Dong-Mei^1,2, MOU Rong³, WU Hui*, LIU Yu-He³, ZHONG Jia-Teng¹, YU Chun-Yan³, YANG Dong-Yan⁴

1.Dpartment of Pathophysiology,Norman Bethune College of Medicine,Jilin Univers
ity|Changchun 130021,China;2.Department of Cardiology,Central Hospital of Changchun,Changchun 130051,China 3.Department of Pathology,School of Basic Medical Sciences,Beihua University,Jilin 132000,China;4.Department of Electrodiagnosis,China-Japan Union Hospital,Jilin University, Changchun 130033,China

Received:2010-08-25 Online:2011-01-28 Published:2011-01-28

摘要/Abstract

摘要：

目的：检测人肾细胞癌（RCC）组织中Notch信号途径相关蛋白的表达水平，探讨Notch信号途径分子与人RCC发生发展的关系。方法：手术方法获得6例经病理检查诊断为RCC组织标本，采用Western blotting法检测RCC组织和癌旁组织中Notch信号通路受体、配体蛋白表达水平。结果：与癌旁组织比较，RCC组织中Notch信号的受体Notch-1以及配体Jagged-1的表达水平均明显降低(P<0.05），受体Notch-2及配体Jagged-2表达水平均明显升高(P<0.05）。结论：RCC中Notch信号途径蛋白表达水平发生了明显的变化，Notch信号途径可能参与了RCC的发生发展过程。

关键词: 肾细胞癌；Notch信号途径；蛋白表达；免疫印迹

Abstract:

Abstract:Objective
To detect the Notch signaling pathway-related protein expression levels in human renal cell carcinoma (RCC) tissues and explore the relationship between Notch signaling pathway molecule and occurrence and development of RCC.Methods 6 cases of RCC diagnosed by pathology detection were obtained by surgical methods.The Notch signaling pathway receptor and ligand protein levels in RCC tissues and adjacent tissues were detected with Western blotting. Results Compared with adjacent tissues, the expression levels of the receptor of Notch signaling Notch-1 and the ligand Jagged-1 in RCC tissues were significantly reduced（P<0.05）,the expression levels of the receptor Notch-2 of the Notch signaling pathway and the ligand Jagged-2 in RCC tissues were significantly increased（P<0.05）.Conclusion The expression of N
otch signaling pathway protein in RCC has apparente change,Notch signaling pathway may be involved in the occurrence and development of RCC.

Key words: renal cell carcinoma;Notch signaling pathway;protein expression;Western blotting

中图分类号:

R737.11

娄冬梅, 牟荣, 武慧, 刘玉和, 钟加滕, 于春艳, 杨冬艳. 肾细胞癌组织中Notch信号途径蛋白表达水平的检测及其意义[J]. J4, 2011, 37(1): 93-96.

LOU Dong-Mei, MOU Rong, WU Hui, LIU Yu-He, ZHONG Jia-Teng, YU Chun-Yan, YANG Dong-Yan. Determination of expression of Notch signaling pathway protein |in renal cell carcinoma tissues and its significance[J]. J4, 2011, 37(1): 93-96.