HIP1在维生素K3诱导Hela细胞损伤中的表达及意义

J4 ›› 2009, Vol. 35 ›› Issue (5): 790-793.

HIP1在维生素K3诱导Hela细胞损伤中的表达及意义

于春艳^1,2, 康劲松¹, 李洪岩¹, 钟加滕¹, 王伟伟¹, 孙连坤¹

1.吉林大学基础医学院病理生理学教研室|吉林长春 130021；2.北华大学基础医学院病理学教研室|吉林吉林132013

收稿日期:2009-04-14 出版日期:2009-09-28 发布日期:2009-09-28
通讯作者: 孙连坤 E-mail:sunlk@jlu.edu.cn
作者简介:于春艳(1975－),女,吉林省吉林市人|在读医学博士,主要从事肿瘤病理生理学研究。
基金资助:
国家自然科学基金资助课题（30570687）;吉林省科技厅医学专项基金资助课题（200505139）

Expression of HIP1 in injuried Hela cells induced by vitamin K3 and significance

YU Chun-Yan^1,2, KANG Jin-Song¹, LI Hong-Yan¹, ZHONG Jia-Teng¹, WANG Wei-Wei¹, SUN Lian-Kun¹

1.Department of Pathphysiology,School of Basic Medical Sciences| Jilin University Changchun 130021,China;2.Department of Pathology|School of Basic Medical Sciences,Beihua University,Jinlin 132013,China

Received:2009-04-14 Online:2009-09-28 Published:2009-09-28

摘要/Abstract

摘要：

目的：观察Huntingtin 相关蛋白1（HIP1）在维生素K3诱导Hela细胞损伤中的表达及意义。方法：对数生长期Hela细胞随机分为对照组(Control)、VK3处理组（VK3）、VK3与NAC联合作用组（VK3 + NAC）及NAC单独应用组(NAC)；MTT法检测各组细胞存活率；Western blotting法检测各组HIP1、NF-κB蛋白表达水平；RT-PCR方法检测各组Cyclin D1 mRNA表达水平。结果：与对照组相比，VK3组Hela细胞存活率降低（P<0.01），HIP1蛋白、NF-κB蛋白表达量降低（P<0.05），Cyclin D1 mRNA表达量明显下降（P<0.05）；与VK3组相比，VK3与NAC联合应用组细胞存活率增加，能够明显拮抗HIP1蛋白，NF-κB蛋白表达量和Cyclin D1 mRNA表达量均增加（P<0.05）。结论：VK3具有抑制细胞增殖作用，可能与降低HIP1的蛋白表达水平有关。

关键词: 维生素K3；Hela细胞；Huntingtin相关蛋白1；核转录因子-κB;细胞周期蛋白D1

Abstract:

Abstract:Objective To observe the expression of Humtingtin interacting protein 1(HIP1) in the injury of Hela cells induced by vitamin K3(VK3).Methods Hela cells in log phase were randomly divided into control group,VK3 group,VK3+NAC group and NAC group,respectively.The survival rate of Hela cells was determined by MTT.The expressions of HIP1 and NF-κB protein in Hela cells were detected by Western blotting.The expression of Cyclin D1 mRNA was tested by RT-PCR assay.Results Compared with control group,the survival rate of Hela cells in VK3 group decreased（P<0.05）;the expressions of HIP1 and NF-κB protein and Cyclin D1 mRNA were all decreased in VK3 group （P<0.05）.Compared with VK3 group,the survival rate of Hela cells in VK3 + NAC group was increased（P<0.05）;the expressions of HIP1 and NF-κB protein and Cyclin D1 mRNA were increased（P<0.05）.Conclusion VK3 has an effect of inhibiting the cell proliferation which may be related with down-regulation of HIP1 protein expression.

Key words: vitamin K3;Hela cells;huntingtin interacting protein　1;nuclear factor kappa B;Cyclin D1

中图分类号:

R363.2

于春艳, 康劲松, 李洪岩, 钟加滕, 王伟伟, 孙连坤. HIP1在维生素K3诱导Hela细胞损伤中的表达及意义[J]. J4, 2009, 35(5): 790-793.

YU Chun-Yan, KANG Jin-Song, LI Hong-Yan, ZHONG Jia-Teng, WANG Wei-Wei, SUN Lian-Kun. Expression of HIP1 in injuried Hela cells induced by vitamin K3 and significance[J]. J4, 2009, 35(5): 790-793.