骨髓间充质干细胞移植对大鼠肝纤维化模型的修复作用

J4 ›› 2009, Vol. 35 ›› Issue (5): 874-876.

• 基础研究 • 上一篇下一篇

骨髓间充质干细胞移植对大鼠肝纤维化模型的修复作用

牛铁兵¹, 陈玉丙², 张立新³

1.吉林省医学期刊社|吉林 |长春 |130061；2.吉林大学第二医院放疗科|吉林长春 130041；
3.吉林省公安厅高速公路公安局法医科|吉林长春 130051

收稿日期:2009-04-15 出版日期:2009-09-28 发布日期:2009-09-28
通讯作者: 陈玉丙 E-mail:yb0707@163.com
作者简介:牛铁兵(1963-),男,吉林省长春市人,副主任医师,主要从事老年性疾病的研究。
基金资助:
吉林省科技厅科研基金资助课题(20080446)

Repairment of marrow derived mesenchymal stem cells on hepatic fibrosis in rats

NIU Tie-Bing¹, CHEN Yu-Bing², ZHANG Li-Xin³

1.Medical Journal Club of Jilin Province,Changchun 130061,China；2.Department of Radiotherapy,Second Hospital,Jilin University,Changchun 130041；3.Department of Forensic Medicine,Public Security Bureau of Motorvay,Public Security Department of Jilin Province,Changchun |130051,China

Received:2009-04-15 Online:2009-09-28 Published:2009-09-28

摘要/Abstract

摘要：

目的：观察骨髓间充质干细胞（MSCs）移植对大鼠肝纤维化模型的修复作用，为MSCs的临床应用提供可靠的理论依据。方法：分离、纯化大鼠MSCs并进行体外培养。用皮下多点注射CCl4制备肝纤维化大鼠模型，将大鼠随机分为3组：正常对照组、模型损伤组和MSCs移植组，每组6只。将MSCs经尾静脉回输到MSCs移植组大鼠体内,30 d取肝脏,观察病理变化,免疫组织化学检测α平滑肌肌动蛋白（α-SMA）的表达。结果：流式细胞术检测体外分离培养的MSCs 93.27%处于G0/G1期，且MSCs表面CD44呈阳性表达；将分离的MSCs输入肝纤维化模型大鼠30 d 后，镜下可见MSCs移植组病理改变较模型损伤组明显减轻，肝小叶结构基本正常，肝血窦基本正常，纤维结缔组织无明显增生；α-SMA染色强度MSCs移植组低于模型损伤组。结论：MSCs在一定程度上可以促进纤维化肝组织的修复。

关键词: 骨髓间充质干细胞；肝纤维化；α-SMA；组织修复

Abstract:

To observe the repairing effect of marrow derived mesenchymal stem cells (MSCs) on hepatic fibrosis in rats，and provide a reliable theoretical basis for further clinical application of MSCs.Methods The MSCs of rats were isolated,purified and cultivated in vitro.The model of hepatic fibrosis induced by CCl4 was established.The rats were divided into three groups:normal control group,model group,MSCs transplantation group,6 each group.The MSCs were transplatated into the rats through tail vein in MSCs transplantation group.The repairing effect of MSCs on hepatic fibrosis was observed and the expression of α-SMA in liver tissue was detected by immunohistochemistry 30 d after transplantation.Results 93.27% MSCs were at G0/G1 phase,and the CD44 surface expression in the MSCs was positive.The hepatic pathological injury changes of rats in MSCs transplantation group were significantly reduced than that in model group 30 d after MSCs injection.The lobular structure and liver sinusoids were normal and hyperplasia of fibrous connective tissue was not obvious.The intensity of α-SMA staining in MSCs transplantation group was lower than that in model group.Conclusion MSCs have repairment on hepatic fibrosis in rats to a certain extent.

Key words: mesenchymal stem cells;hepatic fibrosis;α-SMA；tissue repair

中图分类号:

R329.2

牛铁兵, 陈玉丙, 张立新. 骨髓间充质干细胞移植对大鼠肝纤维化模型的修复作用[J]. J4, 2009, 35(5): 874-876.

NIU Tie-Bing, CHEN Yu-Bing, ZHANG Li-Xin. Repairment of marrow derived mesenchymal stem cells on hepatic fibrosis in rats[J]. J4, 2009, 35(5): 874-876.

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