P33ING1b和KAI1/CD82在贲门癌组织中的表达及其意义

doi:10.7694/jldxyxb20130325

吉林大学学报(医学版) ›› 2013, Vol. 39 ›› Issue (3): 548-553.doi: 10.7694/jldxyxb20130325

P33ING1b和KAI1/CD82在贲门癌组织中的表达及其意义

董良鹏¹，陈玲云²，秦双^1

1.新乡医学院第一附属医院普外科，河南　卫辉　453100；2. 新乡医学院第一附属医院手术室，河南　卫辉　453100

收稿日期:2012-12-17 出版日期:2013-05-28 发布日期:2013-07-01
通讯作者: 董良鹏（Tel:0373-4402411,E-mail:dlipen@yahoo.com.cn） E-mail:dlipen@yahoo.com.cn
作者简介:董良鹏（1978-），男，河南省林州市人，主治医师，医学硕士，主要从事普通外科的基础与临床研究。
基金资助:
河南省卫生厅科技攻关项目资助课题（200804055）

Expressions of P33ING1b and KAI1/CD82 in gastric cardia carcinoma tissue and their significances

DONG Liang-peng¹,CHEN Ling-yun²,QIN Shuang¹

1.Department of General Surgery,First Affiliated Hospital,Xinxiang Medical University,Weihui 453100,China；2.Operating Room,First Affiliated Hospital,Xinxiang Medical University,Weihui 453100,China

Received:2012-12-17 Online:2013-05-28 Published:2013-07-01

摘要/Abstract

摘要： ［摘要］目的：探讨抑癌基因P33INGlb和KAI1/CD82在贲门癌(GCA)组织中的表达及二者在GCA的发生和恶性进展中的作用。方法：选取20例成人正常贲门黏膜（正常组）和51 例GCA组织（GCA组），采用免疫组织化学方法检测2组组织中P33INGlb和KAI1/CD82蛋白表达的阳性率，结合临床病理资料进行统计学分析。结果：GCA和贲门黏膜细胞的胞浆见KAI1/CD82蛋白阳性表达颗粒。正常贲门黏膜组织中KAI1/CD82蛋白表达的阳性率为85.0%，GCA组织中KAI1/CD82蛋白表达的阳性率为65.7%。伴淋巴结转移患者GCA组织中的KAI1/CD82蛋白表达水平明显低于无淋巴结转移者(P<0.05)；侵透浆膜患者的GCA组织中KAI1/CD82蛋白表达水平明显低于未侵及浆膜者(P<0.05)。KAI1/CD82蛋白表达的降低或缺失与肿瘤的临床分期有关联(P<0.05)。GCA和贲门黏膜细胞的胞浆和胞核见P33INGlb蛋白阳性表达颗粒。GCA组织较正常贲门黏膜组织的P33INGlb蛋白阳性表达率降低(P<0.05)；P33INGlb蛋白表达的降低或缺失与肿瘤的临床分期有关联(P<0.05)。结论：KAI1/ CD82 蛋白在GCA组织的表达与肿瘤的临床分期、浸润深度和淋巴结转移有关联；GCA组织中P33INGlb蛋白表达率与肿瘤的临床分期有关；抑癌基因P33INGlb和KAI1/CD82在GCA组织中的表达可作为从分子水平判定人GCA恶性生物学行为的指标。

关键词: 贲门肿瘤, KAI1/CD82, P33INGlb, 蛋白表

Abstract: Abstract:Objective To study the expressions of P33ING1b and KAI1/CD82in gastric cardia carcinoma(GCA) tissue and their roles in the occurrence and malignancy progress of　GCA. Methods 20 cases of adult normal cardia mucosa tissue (normal group) and 51 cases of GCA tissue (GCA group) were selected.Immunohistochemistry method was used to detect the positive expression rates of P33INGlb and KAI1/CD82 in the tissue in two groups. The data combined with the clinicopathological features was analyzed. Results KAI1/CD82 protein mainly expressed in the cytoplasm of GCA cells and cardia mucosal cells. The positive expression rate of KAI1/CD82 protein in normal gastric cardia tissue was 85.0%,and the positive expression rate of KAI1/CD82 protein in GCA tissue was 65.7%.The expression level of KAI1/CD82 protein in GCA tissue with lymph node metastasis was lower than that in GCA tissue without lymph node metastasis(P<0.05). The expression level of KAI1/CD82 protein in GCA tissue with serosa invasion was lower than that in GCA tissue without serosa invasion (P<0.05). The loss or reduction of KAI1/CD82 protein expression had relationships with clinical grade of tumor(P<0.05). P33INGlb protein mainly expressed in the cytoplasm and nucleolus of GCA cells and cardia mucosal cells. The expression level of P33INGlb protein in GCA tissue was lower than that in normal　gastric cardia　mucosa tissue (P<0.05). The loss or reduction of P33INGlb protein expression had relationships with clinical grade of tumor (P<0.05). Conclusion The expression of　KAI1/CD82 protein in GCA tissue has relationship with clinical grade,the depth of tumor invasion,and lymth metastasis.The expression rate of P33INGlb protein in GCA tissue has relationship with the clinical grade of tumor. The expressions of KAI1/CD82 protein and P33INGlb protein can be used as indicators to judge the malignant biological behavior of human GCA at molecular level.

Key words: gastric cardia neoplasms, KAI1/CD82, P33INGlb, protein expression

中图分类号:

R735

董良鹏，陈玲云，秦双. P33ING1b和KAI1/CD82在贲门癌组织中的表达及其意义[J]. 吉林大学学报(医学版), 2013, 39(3): 548-553.

DONG Liang-peng,CHEN Ling-yun,QIN Shuang. Expressions of P33ING1b and KAI1/CD82 in gastric cardia carcinoma tissue and their significances[J]. Journal of Jilin University Medicine Edition, 2013, 39(3): 548-553.

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P33ING1b和KAI1/CD82在贲门癌组织中的表达及其意义

Expressions of P33ING1b and KAI1/CD82 in gastric cardia carcinoma tissue and their significances

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