刺五加皂苷对糖基化终产物损伤内皮细胞的保护作用

J4 ›› 2010, Vol. 36 ›› Issue (6): 1084-1087.

刺五加皂苷对糖基化终产物损伤内皮细胞的保护作用

焦淑萍¹, 倪海镜², 薛丽娟²

1. 北华大学医学院生物化学教研室|吉林吉林 132013；2. 吉林大学中日联谊医院药剂科|吉林长春130033

收稿日期:2010-06-22 出版日期:2010-11-28 发布日期:2010-11-28
通讯作者: 倪海镜(Tel:0431-84995158,E-mail:nihaij9966@126.com) E-mail:nihaij9966@126.com
作者简介:焦淑萍（1961-）|女|吉林省吉林市人|教授|主要从事天然产物研究。
基金资助:
吉林省科技厅科研基金资助课题（200302-05）

Protective effects of Acanthopanax senticosus saponins on endothelial cells injured by advanced glycosylation end products

JIAO Shu-Ping¹, NI Hai-Jing¹, XUE Li-Juan²

1.Department of Biochemistry,Medical College, Beihua University,Jilin 132013,China;2.Department of Pharmacy,China-Japan Union Hospital, Jilin University,Changchun 130033,China

Received:2010-06-22 Online:2010-11-28 Published:2010-11-28

摘要/Abstract

摘要：

目的:研究刺五加叶皂苷(ASS)对糖基化终产物(AGE)作用下人脐静脉内皮细胞(HUVECs)的保护作用及其机制。方法: 体外培养HUVECs，糖孵育法制备糖基化终产物修饰牛血清白蛋白（AGE-BSA）。将细胞分为6组，即正常对照组、实验对照组（BSA组）、损伤组（AGE组）、损伤加入ASS低、中、高浓度组。将200 mg·L^-1 AGE作用于不同浓度(10、50和100 mg·L-1)ASS培养4 h的HUVECs，继续培养24 h，测定各组细胞增殖活力及培养液中血管性假血友病因子（vWF）、一氧化氮（NO）及乳酸脱氢酶（LDH）的含量。结果：与BSA组比较， AGE组HUVECs增殖活力明显降低(P<0.01)，AGE组HUVECs培养液vWF和LDH含量明显增加(P<0.01)，NO水平明显降低(P<0.01)。与AGE组比较，10、50和100 mg·L-1 ASS组HUVEC增殖活力明显增高(P<0.05)，vWF 水平显著减少(P<0.05)，LDH含量明显减少(P<0.05)，NO生成量明显增多(P<0.05)。结论: ASS可抑制AGE对内皮细胞的损伤作用，且可抑制AGE减少NO生成的作用，ASS保护内皮细胞免受AGE损伤的机制可能与增加内皮细胞NO生成量有关。

关键词: 糖基化终产物；内皮细胞；一氧化氮；刺五加皂苷；

Abstract:

To study the protective effect of Acanthopanax senticosus saponins （ASS）on human umbilical vein endothelial cells(HUVECs) injured by advanced glycosylation end products(AGE) and its possible mechanism. Methods AGE-modified bovine serum albumin(AGE-BSA) was prepared by incubating the BSA with a high concentration of glucose.The cultured HUVECs were divided into six groups:control group,BSA group,AGE group,low dose ASS + AGE group,middle dose ASS + AGE group,high dose ASS + AGE group.The HUVECs were cultured with 200 mg·L-1 AGE-BAS for 24 h following pre-incubation of ASS at different concentrations.The HUVECs proliferation activity, the Von Willebrand factor(vWF), lactate dehydrogenase (LDH) and nitric oxi
de (NO) levels were determined. Results Compared with BSA group,the HUVECs proliferation activity in AGE group was significantly reduced(P<0.01),the vWF and LDH levels in AGE group were significantly increased(P<0.01) and the NO level were significantly reduced(P<0.01).Compared with AGE group,the HUVECs proliferation activities in ASS 10,50,100 mg·L^-1 groups were increased(P<0.05).The vWF and LDH levels were markedly decreased(P<0.05). The NO level was increased(P<0.05).Conclusion ASS could protect HUVECs against damage induced by AGE and increase the NO level in the AGE- exposed HUVECs.The mechanism may be related to increasing the NO level in HUVECs.

Key words: glycosylation end products;endothelial cells;nitric oxide;acanthopanax senticosus saponins

中图分类号:

R965.1

焦淑萍, 倪海镜, 薛丽娟. 刺五加皂苷对糖基化终产物损伤内皮细胞的保护作用[J]. J4, 2010, 36(6): 1084-1087.

JIAO Shu-Ping, NI Hai-Jing, XUE Li-Juan. Protective effects of Acanthopanax senticosus saponins on endothelial cells injured by advanced glycosylation end products [J]. J4, 2010, 36(6): 1084-1087.