关键词: 甲型H1N1流感疫苗；季节性流感疫苗；特异性抗体；交叉免疫保护性

Abstract:

To compare of immunological efficacy of H1N1 influenza vaccine 0 d one-dose method and 0,21 d,two-dose method,and observe whether seasonal influenza vaccine has cross protection  for H1N1 influenza virus. Methods H1N1 influenza vaccine with two immunological methods was used to inoculate Balb/c mice.The first,the Balb/c mice were inoculated in 0 d. The second,the Balb/c mice were inoculated in 0 and 21 d. The serum from orbital in 14,21,28,35, 42 d after the first-dose were separated. HI was performed to determine the H1N1-specific serum antibody titers to compare the immune effect of the two immunological methods. Then the Balb/c mice were inoculated with seasonal influenza vaccine. The serum from orbital in 14 and 28 d were separated. HI was performed to determine whether the anti-serum of seasonal influenza vaccine could non-specificly bind with H1N1 influenza virus-specific antigens. Results The 28,35  and 42 d antibody titers of the two-dose method were significantly higher than those of  one-dose methods after the second immunity at the 21th day. And the 42 d antibody titers  of  the two-dose methods were 4 times of that of  one-dose methods. HI was performed to determine the anti-serum of seasonal influenza vaccine,there were  is no H1N1 influenza virus-specific antibodis in it. Conclusion The antibody titers of 0 and 21 d two-dose method still maintain the high level at  28th day,and  are obviously higher than those of one-dose methods. There is no cross-protective immunity between seasonal influenza vaccine and influenza A H1N1 influenza virus.

Key words: H1N1 influenza vaccine;seasonal influenza vaccine;specific serum antibody;cross-protective immunity