不同相对分子质量一年生膜荚黄芪多糖对RAW264.7细胞炎症相关因子表达的影响

J4 ›› 2011, Vol. 37 ›› Issue (6): 1051-1056.

不同相对分子质量一年生膜荚黄芪多糖对RAW264.7细胞炎症相关因子表达的影响

王金虎¹|董娟聪²| 赵继学¹|金顺子²|张海玉¹|张善玉³

1.吉林大学第一医院儿外科|吉林长春 130021；2. 吉林大学公共卫生学院卫生部放射生物学重点实验室|吉林长春 130021；3. 延边大学长白山生物资源与功能分子教育部重点实验室,吉林延吉 133000

收稿日期:2011-06-03 出版日期:2011-11-28 发布日期:2011-11-28
通讯作者: 张海玉（Tel：0431-88782561，E-mail：zhanghaiyu1622@yahoo.com.cn）；张善玉（Tel：0433-2732456，E-mail：syzhang@ybu.edu.cn） E-mail:zhanghaiyu1622@yahoo.com.cn；syzhang@ybu.edu.cn
作者简介:王金虎（1984-）|男|山东省冠县人|在读医学硕士|主要从事天然药物开发研究。
基金资助:
长白山生物资源与功能分子教育部重点实验室资助课题［长生实合字(2008)第05号］

Effects of different molecule weight astragalus polysacharin isolated from annual astragalus membra-neaceus on experssions of inflammatory cytokines in RAW264.7 cells

WANG Jin-hu¹,DONG Juan-cong²,ZHAO Ji-xue¹,JIN Shun-zi²,ZHANG Hai-yu¹,ZHANG Shan-yu³

Received:2011-06-03 Online:2011-11-28 Published:2011-11-28

摘要/Abstract

摘要：

目的：观察不同相对分子质量的一年生膜荚黄芪多糖（APSⅠ）对脂多糖（LPS）诱导的RAW264.7炎症模型分泌促炎因子和抗炎因子的影响，探讨APSⅠ的抗炎作用。方法：体外培养RAW264.7细胞，APSⅠ作用于未经刺激的RAW264.7细胞及LPS（1 mg/L）刺激后RAW264.7细胞，将细胞分为空白对照组、LPS模型组、APSⅠ组及LPS+ APSⅠ不同相对分子质量组(APSⅠ-A，APSⅠ-B，APSⅠ-C)，采用CCK-8法检测不同相对分子质量、不同浓度的APSⅠ组RAW264.7细胞增殖活力，硝酸还原酶法测定细胞上清液中一氧化氮（NO）的含量，酶联免疫吸附法（ELISA）检测细胞上清中肿瘤坏死因子α（TNF-α）、白细胞介素10（IL-10）的分泌量。结果：与空白对照组比较，APSⅠ单独处理组RAW264.7细胞增殖活性降低（P<0.05或P<0.01），NO及IL-10表达水平明显增加（P<0.05），TNF-α的分泌量降低（P<0.05）；与LPS模型组比较， LPS+APSⅠ组RAW264.7细胞增殖活力显著升高（P<0.05或P<0.01），NO、TNF-α的表达明显降低（P<0.05或P<0.01），IL-10的分泌增加（P<0.05或P<0.01）；3种不同相对分子质量APSⅠ之间比较，APSⅠ-C对NO、TNF-α的抑制更为明显，且APSⅠ-C促进IL-10分泌的作用强于APSⅠ-A及APSⅠ-B。结论：APSⅠ　可以拮抗LPS所致的RAW264.7细胞炎症反应；不同相对分子质量APSⅠ的抗炎作用有差异，APSⅠ的生物活性与其相对分子质量存在一定的关联性。

关键词: 黄芪多糖；相对分子质量；RAW264.7细胞；炎症因子

Abstract:

Abstract:Objective To investigate the effects of different molecule weight astragalus polysacharin isolated from the annual astragalus membra-neaceus(APSⅠ)on the expressions of pro-inflammatory and anti-inflammatory cytokine in lipopolysaccharides (LPS)-stimulated RAW264.7 cells,and explore the anti-inflammatory effects of APSⅠ.Methods The RAW264.7 cells were cultivated in vitro,RAW264.7 cells or LPS-induced (1 mg?L-1) RAW264.7 cells were stimulated by APS Ⅰ,then divided into control group,LPS model group,APS Ⅰ group,LPS + different molecule weight APS Ⅰgroups(APS Ⅰ-A,APSⅠ-B,APSⅠ-C).The effects of different molecule weight and different concentrations of APSⅠon the proliferation of RAW264.7 cells were examined by CCK-8 method. The content of NO in the RAW264.7 cells supernatant fluid was detected by means of nitrate reductase.The concentrations of TNF-α and IL-10 in the supernatant fluid was determined with ELISA.Results Compared with control group,the proliferation of RAW264.7 cells was inhibited significantly after treated with APS Ⅰalone (P<0.05 or P<0.01),the levels of NO and IL-10 expressions were increased apparently (P<0.05) while the secretion of TNF-α was decreased (P<0.05);Compared with LPS model group,APS Ⅰ combined with LPS significantly increased the proliferation activity of RAW264.7 cells in LPS + APS Ⅰ groups (P<0.05
or P<0.01),the levels of No and TNF -α expressions were decreased significantly (P<0.05 or P<0.01),and the secretion of IL-10 was increased notably (P<0.05 or P<0.01).The effects of APS varied by different molecular weights,the inhibition of APSⅠ-C on the expressions of NO and TNF-α was more obvious,which had a stronger promotion on the secretion of IL-10 than APSⅠ-A and APS- B.Conclusion APSⅠhas anti-inflammatory effect on the LPS-stimulated RAW264.7 cells,the anti-inflammatory activation of APSⅠvaries by different molecule weights.APSⅠwith small molecule weight has more significant activation.So the biological activity of APSⅠmay be associated with molecule weight.

Key words: astragalus polysacharin;molecule weight;RAW264.7 cells;inflammatory cytokines

中图分类号:

R285.5

王金虎|董娟聪|赵继学|金顺子|张海玉|张善玉. 不同相对分子质量一年生膜荚黄芪多糖对RAW264.7细胞炎症相关因子表达的影响[J]. J4, 2011, 37(6): 1051-1056.

WANG Jin-hu,DONG Juan-cong,ZHAO Ji-xue,JIN Shun-zi,ZHANG Hai-yu,ZHANG Shan-yu. Effects of different molecule weight astragalus polysacharin isolated from annual astragalus membra-neaceus on experssions of inflammatory cytokines in RAW264.7 cells[J]. J4, 2011, 37(6): 1051-1056.

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