HSP65-MUC1肿瘤疫苗对小鼠胰腺组织的分子模拟作用

J4 ›› 2011, Vol. 37 ›› Issue (1): 15-17.

HSP65-MUC1肿瘤疫苗对小鼠胰腺组织的分子模拟作用

孙琳¹, 吴秀丽², 王丽颖², 于永利³

（1 .吉林大学化学学院表面与界面化学重点实验室,吉林长春 130012;2 .吉林大学白求恩医学院分子生物学教研室,吉林长春 130021;3 .吉林大学白求恩医学院免疫学教研室,吉林长春 130021）

收稿日期:2010-01-05 出版日期:2011-01-28 发布日期:2011-01-28
通讯作者: 于永利 E-mail:ylyu@mail.jlu.edu.cn
作者简介:孙琳(1976-)|女|辽宁省大连市人|讲师|医学博士|主要从事生物医学材料的应用研究。
基金资助:
家重点基础研究发展计划(973计划)项目资助课题(2001CB510007)

Molecular mimicry of murine pancreas by HSP65-MUC1

SUN Lin¹, WU Xiu-Li², WANG Li-Ying², YU Yong-Li³

(1.Key Laboratory of Surface and Interface Chemistry of Jilin Province,College of Chemistry,Jilin University,Changchun 130012,China;2.Department of Molecular Biology|Norman Bethune College of Medicine|Jilin University|Changchun 130021|China;3. Department of Immunology|Norman Bethune College of Medicine|Jilin University|Changchun 130021|China)

Received:2010-01-05 Online:2011-01-28 Published:2011-01-28

摘要/Abstract

摘要：

目的：探讨重组抗肿瘤融合蛋白疫苗热激蛋白65-黏蛋白1（HSP65-MUC1）在小鼠体内通过分子模拟方式损伤小鼠胰腺的可能性。方法：21只C57BL/6小鼠随机分为PBS对照组、HSP65组和HSP65-MUC1组(每组7只)，分别皮下注射PBS、HSP65和HSP65-MUC1，每周1次，给药3周。无菌分离小鼠脾细胞，利用流式细胞术检测特异性淋巴细胞的增殖情况，组织病理学观察小鼠胰腺的病理改变。结果：流式细胞术检测，与PBS对照组和HSP65组比较，HSP65-MUC1肿瘤疫苗组HSP65-MUC1特异性淋巴细胞升高了16.88%（P<0.001）；HSP65特异性淋巴细胞升高了7.29%（P<0.01）；与HSP60交叉识别的特异性淋巴细胞升高了5.79%（P<0.05）。3个免疫组小鼠胰腺组织HE染色病理均正常。结论：HSP65-MUC1肿瘤疫苗没有通过分子模拟诱导损伤小鼠胰腺。

关键词: 重组抗肿瘤融合蛋白；疫苗；热激蛋白65-黏蛋白1；分子模拟

Abstract:

Abstract:Objective To explore the possibility to damage mice pancreas by recombinant fusion protein vaccine,heat shock protein 65-mucin 1 (HSP65-MUC1) via molecular mimicry.Methods 21 C57BL/6 mice were randomly divided into three groups:PBS control,HSP65 and HSP65-MUC1 groups.The mice in each group were injected underneath with PBS,HSP65 and HSP65-MUC1, respectively,once a week for three weeks. The mouse pancreas were isolated under aseptic situation,the proliferation of specific lymphocytes was tested by flow cytometry,the pathological changes of mouse pancreas were detected with histo-pathological analysis. Results By flow cytometry,compared with PBS and HSP65 groups,the lymphocytes specific to HSP65-MUC1 of mice in HSP65-MUC1 group were increased by 16.88%（P<0.001）,the lymphocytes specific to HSP65 were increased by 7.29 %（P<0.01）;the lymphocytes specific to HSP60 were increased by 5.79 %（P<0.05). However,HE staining of mouse pancreas in immunized mice kept normal. Conclusion The mouse pancreas are not injured by HSP65-MUC1 via molecular mimicry.

Key words: recombinant fusion protein anti-tumor;vaccines;heat shock protein 65-mucin1;molecular mimicry

中图分类号:

R392.11

孙琳, 吴秀丽, 王丽颖, 于永利. HSP65-MUC1肿瘤疫苗对小鼠胰腺组织的分子模拟作用[J]. J4, 2011, 37(1): 15-17.

SUN Lin, WU Xiu-Li, WANG Li-Ying, YU Yong-Li. Molecular mimicry of murine pancreas by HSP65-MUC1[J]. J4, 2011, 37(1): 15-17.