关键词: 3,4,5-三羟基苯甲酸；抗肿瘤药；抗氧化剂

Abstract:

Abstract:Objective  To observe the inhibitory effect of 3,4,5-hydroxy benzoic acid (TBA) on H₂₂ transplanted tumor in mice and  its influence in survival time and antioxidant capacity in vivo of H₂₂ tumor bearing mice .Methods H₂₂  tumor bearing mouse models were established.The H₂₂ tumor bearing mice were randomly divided into  control group,positive control group,TBA low dose group,TBA middle dose group,and TBA high dose group.There were 10 mice in each group.0.5% sodium carboxymethyl cellulose solution 2 mL·10 g^-1 was administered by oral in control group.CTX 20 mg·kg^-1 was injected intraperitoneally in positive control group.TBA was administered by oral in TBA low (0.13 g·kg^-1),middle(0.25 g·kg^-1) and high(0.50 g·kg^-1)dose groups.The H₂₂  tumor bearing mice were administered once a day at fixed time and  lasted  for 10 d.Then,the tumor  weight,the survival time,and  the SOD activity,MDA and GSH levels in serum in each group were detected.Results Compared with control group,the average tumor weights were  reduced in TBA middle dose group and CTX group (P<0.05)，there was no significant difference between other groups;compared with control group,the mean survival time in TBA middle dose group and CTX group  were longer (P<0.05),there was no significant difference between other groups;compared with control group,the SOD activities  in TBA middle and high dose groups were significantly increased(P<0.05);the MDA level  in TBA middle dose group was significantly decreased(P<0.05);the GSH levels were in TBA low,middle,high dose groups were significantly increased(P<0.05). Conclusion  TBA could inhibit the growth of H₂₂  tumor  and prolong the survival time of tumor bearing mice by oral administration. TBA could clear the free radicals in tumor bearing mice and up-regulate the  oxidative stress status of body.

Key words: 3,4,5-trihydroxybenzoic acid;antineoplastic agents;antioxidants