嘌呤核苷酸对海洛因依赖及戒断大鼠睾丸和附睾组织中腺苷脱氨酶活性及基因表达的影响

J4 ›› 2010, Vol. 36 ›› Issue (2): 291-294.

嘌呤核苷酸对海洛因依赖及戒断大鼠睾丸和附睾组织中腺苷脱氨酶活性及基因表达的影响

崔佳乐^1,2, 何海涛², 洪新雨³, 邓志会², 赵丽艳⁴, 洪敏²

1.吉林大学基础医学院组织学与胚胎学教研室|吉林长春 130021;2.吉林大学基础医学院生物化学与分子生物学教研室|吉林长春 130021;3.吉林大学第一医院神经外科|吉林长春130021;4. 吉林大学第二医院检验科|吉林长春 130041

收稿日期:2009-10-14 出版日期:2010-03-28 发布日期:2010-03-28
通讯作者: 洪敏（Tel:0431-85619757， E-mail:hongmin@email.jlu.edu.cn） E-mail:hongmin@email.jlu.edu.cn
作者简介:崔佳乐（1978-）|女|黑龙江省齐齐哈尔市人|讲师|医学博士|主要从事药物依赖及生殖内分泌研究。
基金资助:
吉林省科技厅科研基金资助课题（200080435-2）；吉林大学科研基金资助课题（2002QD001）

Effect of purine nucleotide on adenosine deaminase activity and gene expression in testis and epididymis tissues of heroin dependence and withdrawal rats

CUI Jia-yue^1,2, HE Hai-Tao², HONG Xin-Yu³, DENG Zhi-Hui², ZHAO Li-Yan⁴, HONG Min²

1.Department of Histology and Embryology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China;2.Department of Biochemistry and Molecular Biology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China;3.Department of Neurosurgery,First Hospital,Jilin University,Changchun 130021,China;4 Department of Laboratory,Second Hospital,Jilin University,Changchun 130041,China

Received:2009-10-14 Online:2010-03-28 Published:2010-03-28

摘要/Abstract

摘要：

目的：研究嘌呤核苷酸对海洛因依赖及戒断大鼠睾丸、附睾腺苷脱氨酶(ADA)活性及基因表达的影响，阐明海洛因对男性生殖系统嘌呤核苷酸代谢的损害及嘌呤核苷酸补偿的对抗作用。方法：建立海洛因依赖及戒断大鼠模型，80只建模成功的雄性Wistar大鼠随机分为对照组（生理盐水）、海洛因组、海洛因戒断3 d组、海洛因戒断9 d组、核苷酸组（不给海洛因）、海洛因+核苷酸组（同时给药）、核苷酸3 d组及核苷酸9 d组（每组10只），检测睾丸和附睾组织内ADA活性及基因表达的情况。结果：睾丸和附睾组织中ADA活性，海洛因组和海洛因戒断3 d组均明显高于对照组（P<0.05），其他各组与对照组比较，差异均无显著性（P>0.05）；睾丸组织中ADA mRNA水平，海洛因组明显高于对照组（P<0.05），附睾组织中ADA mRNA水平，海洛因组、海洛因戒断3 d组和海洛因戒断9 d组均明显高于对照组（P<0.05），其他各组与对照组比较，差异均无显著性（P>0.05）。结论：海洛因对大鼠睾丸和附睾组织中ADA活性和基因表达有持续增强作用，补偿嘌呤核苷酸可以对抗海洛因的作用。

关键词: 海洛因；嘌呤核苷酸类；腺苷脱氨酶；睾丸；附睾

Abstract:

Abstract:Objective To investigate the influence of purine nucleotide on adenosine deaminase (ADA) activity and gene expression in testis and epididymis tissues of heroin dependence and withdrawal rats.Methods The rat models of heroin dependence and withdrawal were set up.80 male Wistar rats were randomly divided into 8 groups (10 rats of each group):control group(C),nucleotide group(N),heroin administration group(H),heroin and nucleotide group(HN),3 d withdrawal group(W3),9 d withdrawal group(W9),nucleotide 3 d group(N3),nucleotide 9 d group(N9).The adenosine deaminase activity and gene expression in testis and epididymis tissues of rats in various groups were detected.Results Compared with control group,the ADA activities in heroin administration group and 3 d withdrawal group in testis and epididymis tissues were increased significantly (P<0.05);there was no obvious difference of ADA activity between other groups and control group（P>0.05）.The ADA mRNA level in testis tissue in heroin group was significantly higher than that in control group (P<0.05);the ADA mRNA levels in epididymis tissue in heroin group,3 d withdrawal group and 9 d withdrawal group were significantly higher than that in control group (P<0.05);and there was no obvious difference of ADA mRNA level between other groups and control group（P>0.05）.Conclusion Heroin can enhance constantly the adenosine deaminase activity and gene expression in testis and epididymis tissues of rats.Purine nucleotide compensation can oppose the effect of heroin.

Key words: heroin；purine nucleotides；adenosine deaminase；testis；epididymis

中图分类号:

R996.5

崔佳乐, 何海涛, 洪新雨, 邓志会, 赵丽艳, 洪敏. 嘌呤核苷酸对海洛因依赖及戒断大鼠睾丸和附睾组织中腺苷脱氨酶活性及基因表达的影响[J]. J4, 2010, 36(2): 291-294.

CUI Jia-yue, HE Hai-Tao, HONG Xin-Yu, DENG Zhi-Hui, ZHAO Li-Yan, HONG Min. Effect of purine nucleotide on adenosine deaminase activity and gene expression in testis and epididymis tissues of heroin dependence and withdrawal rats[J]. J4, 2010, 36(2): 291-294.