小寡核苷酸对大鼠骨髓间充质干细胞向成骨细胞分化的影响

J4 ›› 2010, Vol. 36 ›› Issue (2): 336-339.

小寡核苷酸对大鼠骨髓间充质干细胞向成骨细胞分化的影响

申玉芹¹, 孙新华², 于丽³, 王学菊⁴, 冯志远², 王丽颖⁵, 于永利⁶

1.吉林大学口腔医院牙周病科|吉林长春 130021；2.吉林大学口腔医院正畸科|吉林长春 130021；3.黑龙江省口腔病防治院正畸科|黑龙江哈尔滨 150001；4.吉林大学中日联谊医院病理科|吉林长春 130033；5. 吉林大学基础医学院分子生物学教研室|吉林长春 130021；6.吉林大学基础医学院免疫学教研室|吉林长春 130021

收稿日期:2009-12-15 出版日期:2010-03-28 发布日期:2010-03-28
通讯作者: 孙新华(Tel：0431-88796023,E-mail：xinhuasun8@163.com) E-mail:xinhuasun8@163.com
作者简介:申玉芹(1970-),女,吉林省白山市人| 主治医师|在读医学博士|主要从事牙周组织改建的临床与基础研究。
基金资助:
吉林省科技厅白求恩医学专项基金资助课题（200705344）

Effect of oligodeoxynucleotide on differentiation of rat bone marrow mesenchymal stem cells to osteoblasts

SHEN Yu-Qin¹, SUN Xin-Hua², YU Li³, WANG Xue-Ju⁴, FENG Zhi-Yuan², WANG Li-Ying⁵, YU Yong-Li⁶

1.Department of Periodontology,Stomatology Hospital,Jilin University,Changchun 130021,China;2.Department of Orthodontics,Stomatology Hospital,Jilin University,Changchun 130021,China;3.Department of Orthodontics,Heilongjiang Dentist Disease Prevention and Treatment Hospital,Harbin 150001,China；4.Department of Pathology,China-Japan Union Hospital,Jilin University,Changchun 130033|China；5.Department of Molecular Biology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China；6.Department of Immunology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China

Received:2009-12-15 Online:2010-03-28 Published:2010-03-28

摘要/Abstract

摘要：

目的：筛选具有促进大鼠骨髓间充质干细胞（BMSCs）成骨分化作用的小寡核苷酸（ODN），探讨ODN对BMSCs向成骨细胞分化的影响。方法：取第3代BMSCs孵育24 h后进行成骨诱导，双盲法加入12条不同的ODN，PBS作为溶媒对照组，应用碱性磷酸酶(ALP)试剂盒检测ODN于不同工作浓度（4.0、 2.0、 1.0和 0.5 mg/L）、不同时间点（24、72和120 h）刺激经成骨诱导后的BMSCs内ALP的表达水平，筛选出具有促成骨分化作用的ODN。结果：与PBS溶媒对照组比较，ODN工作浓度为4.0和0.5 mg/L时，实验组与对照组ALP表达水平差异无显著性(P>0.05)，ODN工作浓度为2.0和1.0 mg/L时，有2条ODN在不同时间点（24、72和120 h）均可促进BMSCs内ALP的表达（P<0.01）。结论：在12条不同的ODN中共筛选出2条具有促进BMSCs成骨分化作用的ODN，表明特定序列的ODN能够影响大鼠BMSCs向成骨细胞的分化。

关键词: 骨髓间充质干细胞；分化；小寡核苷酸；成骨细胞

Abstract:

Abstract:Objective To investigate the effect of oligodeoxynucleotide (ODN) on differentiation of rat bone marrow mesenchymal stem cells(BMSCs) to osteoblasts.Methods BMSCs at the third generation were incubated for 24 h before 12 different ODN were added.The levels of alkaline phosphatase(ALP),reflecting the degree of osteogenic differentiation were detected in four replicate wells of BMSCs stimulated with each ODN with different concentrations( 4.0,2.0,1.0 and 0.5 mg/L) at different time points(24,72 and 120 h).Results There were no significant differernces of the mean level of ALP between 4.0 and 0.5 mg/L ODN groups and PBS control group;but in 2.0 and 1.0 mg/L ODN groups,there were two ODN which could increase the ALP levels at different time points(24,72 and 120 h)(P<0.01). Conclusion Two ODN of 12 different ODN can promote the diffentiation of BMSCs to osteoblasts.It suggests that ODN with special sequence characteristic can significantly promote the differentiation of rat BMSCs to osteoblasts.

Key words: bone marrow mesenchymal stem cells；differentiation ；oligodeoxynucleotide；osteoblasts

中图分类号:

R329.2

申玉芹, 孙新华, 于丽, 王学菊, 冯志远, 王丽颖, 于永利. 小寡核苷酸对大鼠骨髓间充质干细胞向成骨细胞分化的影响[J]. J4, 2010, 36(2): 336-339.

SHEN Yu-Qin, SUN Xin-Hua, YU Li, WANG Xue-Ju, FENG Zhi-Yuan, WANG Li-Ying, YU Yong-Li. Effect of oligodeoxynucleotide on differentiation of rat bone marrow mesenchymal stem cells to osteoblasts[J]. J4, 2010, 36(2): 336-339.

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