大孔树脂纯化苦碟子总黄酮的工艺条件和参数

J4 ›› 2011, Vol. 37 ›› Issue (5): 792-796.

大孔树脂纯化苦碟子总黄酮的工艺条件和参数

吴金义^1,2, 李兴杰², 陈雪松², 董雪莲², 邱智东²

1.吉林大学中日联谊医院心内科|吉林长春　130033；2.长春中医药大学药学院|吉林长春 130117)

收稿日期:2011-06-29 出版日期:2011-09-28 发布日期:2011-09-28
通讯作者: 邱智东 E-mail:（Tel：0431-86172786，E-mail: qzdcczy@163.com）
作者简介:吴金义（1963-）|男|山东省寿光市人|副教授|医学硕士|主要从事心血管疾病基础及临床研究。
基金资助:
卫生部“重大新药创制”科技重大专项项目资助课题（2009ZX09102-115）

Process conditions and parameters of purification of total flavonoid of China ixeris by macroporous resin

WU　Jin-Yi^1,2, LI Xin-Jie²,CHEN Xue-Song², DONG Xue-Lian², QIU Zhi-Dong²

（1.Department of |Cardiology, China-Japan Union Hospital,Jilin University,Changchun |130033,China;2.School of Pharmacy,Changchun University of Chinese Medicine, Changchun 130117,China）

Received:2011-06-29 Online:2011-09-28 Published:2011-09-28

摘要/Abstract

摘要：

目的：探讨大孔树脂富集纯化苦碟子总黄酮的工艺条件和参数，以提高三苦滴丸的质量，降低三苦滴丸单服剂量。方法：以苦碟子总黄酮获得量为考察指标，采用紫外-分光光度计法测定不同类型大孔树脂(D101、AB-8、DM130、D312和NKA)纯化三苦滴丸中苦碟子总黄酮的工艺条件。结果:最佳纯化工艺为采用 DM130大孔树脂，提取液以（8 000 r•min^-1 离心40 min，70%乙醇洗脱；苦碟子总黄酮纯度可达65%。结论：采用高速离心及大孔树脂相结合的方法可较好地富集纯化苦碟子中黄酮类成分。

关键词: 三苦滴丸；大孔树脂；纯化；苦碟子总黄酮

Abstract:

Abstract:Objective
To discuss the process conditions and parameters of macroporous resin in enrichment and purification of total flavone in order to improve the product quality of SanKu dropping pills and reduce the single dose of product.Methods The total flavonoid of China ixeris were obtained as the quantity indexes. The process conditions of different types of macroporous resin D101,AB-8,DM130,D312 and NKA in purification of total flavonoid were detected by UV. Results The best purification process was DM130 macroporous resin,centrifugal of the extract at 8 000 r•min^-1,for 40 min, 70% ethanol elution.So the total flavonoid could reach 65%.Conclusion High speed centrifugal combined with macroporous resin is good for enriching total flavonoid of Sanku dropping pills.

Key words: Sanku dropping pills;macroporous resin;purification;total flavonoids of Ixeris sonchifolia

中图分类号:

R284

吴金义, 李兴杰, 陈雪松, 董雪莲, 邱智东. 大孔树脂纯化苦碟子总黄酮的工艺条件和参数[J]. J4, 2011, 37(5): 792-796.

WU　Jin-Yi, LI Xin-Jie, CHEN Xue-Song, DONG Xue-Lian, QIU Zhi-Dong. Process conditions and parameters of purification of total flavonoid of China ixeris by macroporous resin[J]. J4, 2011, 37(5): 792-796.

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