关键词: 间充质干细胞, flt-1, KDR, 显微镜检 查, 共焦, 内皮生长因子, 逆转录聚合酶链反应

Abstract: Objective To investigate the expressions of vascular endothelial growth factor (VEGF) receptors flt-1 and KDR in human mesenchymal stem cells (MSCs). Methods MSCs were separated from bone marrow with Percoll reagent and expanded in culture medium. The surface antigens were detected, the labelled cells were analysed on a FACScan flow cytometer. The flt-1 and KDR expressi ons in MSCs were observed under laser scanning confocal microscope (LSCM). RT-PCR was used to detect the changes of flt-1 and KDR expressions treated with VEGF. Results Cultured MSCs were expanded as undifferentiated cells and the purity reached 95%. VEGF receptors flt-1 and KDR were positive in MSCs and the positive substance lied in cytoplasm and membrane of the cells. In MSCs treated with VEGF, the expression of flt-1 mRNA was increased, and the expression of KDR mRNA had not significant change, compared with control group.

Key words: mesenchymal stem cells, flt-1, KDR, microscopy, confocal, endothelial growth factors, reverse transcript ase polymerase chain reaction