关键词: CD4基因；慢病毒；Jurkat细胞；293T细胞；启动子

Abstract:

Abstract:Objective
To study the effect of heterogeneous gene promoter on regulation of exogenous gene expression   in Jurkat cells and provide the theoretical basis for establishment of leukemia animal model.Methods The pig CD4 gene promoter was focasted and cloned by bioinformatics means;then using the cloned CD4 genen promoter,the lentivirus package was performed and the lentiviral vector was constructed.Affter the packaged virus was obtained the titer of virus was detected and the 293 T cells and Jurkat cells were infected to perform green fluorescent protein detection.The expressions  of exogenous genes of the pigs CD4 gene promoter and EF-1α promoter were compared.Results The pig CD4 gene promoter was successfully forecasted and the lentiviral vector was successfully constructed and the virus was packaged out.The dection of titer of virus and infection of 293 T cells and Jurkat cells showed that the start effect of GFP of two promoters in the cells were similar.Conclusion The pig CD4 gene promoter has not specific start of exogenous genes in leukemia Jurkat cells.The pig CD4 gene promoter can serve as a kind of different choice start of heterologous gene in leukemia Jurkat cells.

Key words: CD4 gene;lentiviral;Jurkat cells;293T cells;promoter