氟西汀对创伤后应激障碍大鼠海马组织5-HT1A受体表达的影响

J4 ›› 2012, Vol. 38 ›› Issue (4): 674-677.

氟西汀对创伤后应激障碍大鼠海马组织5-HT1A受体表达的影响

徐爱军¹|刘继刚²|刘昊³|王海涛¹|李冉¹

1. 河北联合大学基础医学院组织学与胚胎学教研室|河北唐山 063000；2. 河北省遵化市人民医院|河北遵化 064200；3. 河北联合大学附属医院神经内科|河北唐山 063000

收稿日期:2012-02-24 出版日期:2012-07-28 发布日期:2012-07-28
通讯作者: 王海涛（Tel：0315-3725754， E-mail：wht92725@163.com） E-mail:wht92725@163.com
作者简介:徐爱军（1978-）|女|河北省秦皇岛市人|讲师|医学硕士|主要从事应激性精神疾病发病机制研究。
基金资助:
河北省科技厅自然科学基金资助课题（H2012401009）;河北省卫生厅医学科学研究重点项目资助课题（20110524，20100469）；河北省唐山市科学技术研究与发展计划项目资助课题（10140201A-1）

Effect of fluoxetine on expression of 5-HT type 1A receptor in hippocampus tissue of posttraumatic stress disorder rats

XU Ai-jun¹,LIU Ji-gang²,LIU Hao³,WANG Hai-tao¹,LI Ran¹

1. Department of Histology and Embryology,College of Basic Medical Sciences,Hebei United University,Tangshan 063000,China|2. Zunhua People’s Hospital,Zunhua 064200,China;3. Department of Neurology,Affiliated Hospital,Hebei United University,Tangshan 063000,China

Received:2012-02-24 Online:2012-07-28 Published:2012-07-28

摘要/Abstract

摘要：

目的：研究创伤后应激障碍（PTSD）大鼠海马组织中5-羟色胺受体1A（5-HT1A受体）表达变化，探讨氟西汀治疗PTSD的机制。方法：随机将雄性Wistar大鼠分为对照组、模型组和氟西汀组，采用改良的单一连续应激（SPS & S）方法刺激大鼠建立PTSD大鼠模型，氟西汀组大鼠每天腹腔注射氟西汀（10 mg/kg）治疗。采用免疫印迹法检测大鼠海马组织5-HT1A受体表达，RT-PCR方法检测5-HT1A mRNA的表达。结果：对照组、模型组及氟西汀组大鼠海马组织中5-HT1A相对表达分别为1.18±0.12、0.39±0.05和0.71±0.09，模型组和氟西汀组低于对照组（P<0.05），氟西汀组高于模型组（P<0.05）；对照组、模型组及氟西汀组大鼠海马组织中5-HT1A mRNA表达的积分光密度分别为0.95±0.12、0.19±0.050.74±0.05，模型组和氟西汀组低于对照组（P<0.05），氟西汀组高于模型组（P<0.05）。结论：PTSD大鼠海马组织5-HT1A受体表达减弱，应用氟西汀后可逆转这种现象，提示氟西汀可能通过逆转5-HT1A受体表达降低发挥治疗作用。

关键词: 创伤后应激障碍；海马；5-羟色胺受体；氟西汀

Abstract:

Abstract:Objective To investigate the effect of fluoxetine on the expression of 5-HT type 1A (5-HT1A) receptor in hippocampus tissue of posttraumatic stress disorder (PTSD) rats and to explore the mechanism of fluoxetine in the treatment of PTSD.Methods The male Wistar rats were randomly divided into control group,model group and fluoxetine group.The rat PTSD model was set up using SPS & S method.Hippocampus tissue was isolated and the expression of 5-HT1A receptor wasdetected with Western blotting and RT-PCR.Results The 5-HT1A receptor protein expressions in hippocampus tissue of rats in control group,model group,and flouxetine group were 1.18±0.12,0.39±0.05,and 0.71±0.09,respectively. The 5-HT1A mRNA expressions were 0.95±0.12,0.19±0.05,and 0.74±0.05 in three group,respectively. There were significant differences between model group,flouxetine group and control group (P<0.05).Compared with model group,the 5-HT1A mRNA in flouxetine group was increased (P<0.05).The index was inhibited after treatment with furoxetin. Conclusion Fluoxetine could inhibit the 5-HT1A receptor expression in hippocampus of PTSD rats.

Key words: posttraumatic stress disorder;hippocampi;5-hydroxytryptamine receptor;fluoxetine

中图分类号:

R332.81

徐爱军|刘继刚|刘昊|王海涛|李冉. 氟西汀对创伤后应激障碍大鼠海马组织5-HT1A受体表达的影响[J]. J4, 2012, 38(4): 674-677.

XU Ai-jun,LIU Ji-gang,LIU Hao,WANG Hai-tao,LI Ran. Effect of fluoxetine on expression of 5-HT type 1A receptor in hippocampus tissue of posttraumatic stress disorder rats[J]. J4, 2012, 38(4): 674-677.