高效液相色谱法测定保元清血颗粒中黄芪甲苷含量方法的建立及评价

J4 ›› 2012, Vol. 38 ›› Issue (4): 805-808.

高效液相色谱法测定保元清血颗粒中黄芪甲苷含量方法的建立及评价

孙聪¹|韩业超²|吴强³

1.长春中医药大学基础医学院生物化学与分子生物学教研室|吉林长春 130117；2.吉林省兽药饲料监察所|吉林长春 130062；3.吉林大学中日联谊医院中医科|吉林长春 130033

收稿日期:2012-03-01 出版日期:2012-07-28 发布日期:2012-07-28
通讯作者: 吴强（Tel：0431-84995840 ，E-mail：zap1210@163.com ） E-mail:zap1210@163.com
作者简介:孙聪（1970-）|女|吉林省长春市人|副教授|理学博士|主要从事病理生理学研究。
基金资助:
吉林省科技厅社会发展重点项目资助课题（20110950）；吉林省中医药管理局科技项目资助课题（2010076）

Establishment and evaluation of determination of astragaloside Ⅳ in Baoyuanqingxue granules by HPLC

SUN Cong ¹, HAN Ye-chao², WU Qiang³

1.Department of Biochemistry and Molecular Biology,School of Basic |Medical Sciences,Changchun University of Chinese Medicine,Changchun 130117,China;2.Jilin Institute for Veterinary Drug and Feed Control,Changchun 130062,China;3. Department of Chinese Medicine,China-Japan Union Hospital,Jilin University, Changchun 130033,China

Received:2012-03-01 Online:2012-07-28 Published:2012-07-28

摘要/Abstract

摘要：

目的：探讨影响保元清血颗粒中黄芪甲苷含量测定的各种因素，分析黄芪甲苷含量测定的提取方法及色谱条件，建立黄芪甲苷含量测定的有效方法。方法：保元清血颗粒中黄芪甲苷含量测定前处理采用甲醇超声提取法，检测采用高效液相色谱（HPLC）法，色谱柱为SB-C18柱（Agilent公司生产，ZORBAX，4.6 mm×150 mm，5 μm），流动相为乙腈-水（36∶64），流速为1.00 mL?min-1，柱温为30℃，进样量为20 μL，UV检测器检测波长为203 nm。
结果：在本色谱条件下，黄芪甲苷与其他峰分离良好，在0.010 03～0.200　60　g.L^-1 范围内呈良好的线性关系（r=0.999　9)；平均加样加收率为98.4%，RSD为0.8%（n=6）。结论：采用高效液相色谱法-紫外检测器（HPLC-UV）测定保元清血颗粒中黄芪甲苷含量，方法便捷、准确，可作为黄芪甲苷含量测定的质量控制方法。

关键词: 保元清血颗粒；黄芪甲苷；高效液相色谱法-紫外检测器

Abstract:

Objective To examine the various factors that affect determination of astragaloside Ⅳ in Baoyuanqingxue granules,elucidate the extraction method and chromatographic conditions of determination of astragaloside Ⅳ, and to establish an effective method for determination of astragaloside Ⅳ. Methods High performance liquid chromatography(HPLC) method was used.The SB-C18 column (Agilent，ZORBAX，4.6 mm×150 mm，5 μm)was adopted,the mobile phase was acetonitrile-warer(36∶64) at the flow rate of 1.00 mL?min-1 with UV detection at 203 nm,the volume of injection was 20 μL and the column temperature was 30℃. Results The resolution between astragaloside Ⅳ and other peaks was good. The calibration curve was linear in the range of 0.010 03-0.200 60　g?L-1（r=0.999 9).The average recovery(n=6) of astragaloside Ⅳ was 98.4% with RSD of 0.8%. Conclusion The HPLC-UV method to detect the cotent of astragaloside Ⅳ in Baoyuanqingxue granules is simple and accurate. It can be used for quality control of astragaloside Ⅳ in Baoyuanqingxue granules.

Key words: Baoyuanqingxue granules;astragaloside Ⅳ;high performance liquid chromatography -UV

中图分类号:

Q503

孙聪|韩业超|吴强. 高效液相色谱法测定保元清血颗粒中黄芪甲苷含量方法的建立及评价[J]. J4, 2012, 38(4): 805-808.

SUN Cong, HAN Ye-chao, WU Qiang. Establishment and evaluation of determination of astragaloside Ⅳ in Baoyuanqingxue granules by HPLC[J]. J4, 2012, 38(4): 805-808.