关键词: 免疫酶细胞化学, 免疫荧光细胞化学, 足细胞, 尿液

Abstract:

Objective To compare the effects of  immunofluorescence and immunoenzymatic celluar chemistry in detecting the urinary podocytes of I
gA nephropathy (IgAN) patients and to provide  basis for selecting the detection method of  urinary podocytes.
Methods 200 mL morning urine samples were collected from 43 cases who diagnosed as IgAN  (experiment group) and 10 cases of normal control subjects (control
 group) for 3 consecutives days before renal biopsy,and the smears were made from the supernatant by centrifugation.The IgAN patients were classified into Lee Ⅰ
 -Lee Ⅴ groups according to Lee degree method.Immunofluorescence and immunoenzymatic celluar chemistry with monoclonal anti-podocalyxin(PCX) antibody were performed to detect the  urinary podocytes and the number of urinary podocytes was counted  by fluorescence and optical microscope;the correlation between the number of urinary podocytes detected by two kinds of methods were analyzed by  Spearman correlation analysis.
Results There were no urinary podocytes of the objects in   control group detected by  two kinds of  methods,but in
  experiment group they were  visible;the positive rate and the median of  urinary  podocytes detected  by immunofluorescence celluar chemistry were lower than those detected  by immunoenzymatic celluar chemistry  (P<0.05);there was a positive  correlation between two kinds of method
s（r=0.842，P<0.01）.Conclusion Immunoenzymatic celluar chemistry is more effective compared with  immunofluorescence celluar chemistry in detecting the  urinary podocytes.
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Key words: immunoenzymatic celluar chemistry, immunofluorescence celluar chemistry, podocyte, urine