吉林大学学报(医学版)

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伪人参皂苷GQ对阿霉素致大鼠急性心肌损伤的保护作用

金鑫1，沈卫章1，金立方1，贾姣源1，李小丰1，王秀丽1，邸 鑫*，张红娟*，李平亚2   

  1. 1. 吉林大学第二医院肿瘤血液科，吉林 长春 130041； 2. 吉林大学再生医学科学研究所，吉林 长春 130021
  • 收稿日期:2013-11-02 发布日期:2013-12-12
  • 通讯作者: 李平亚（Tel：0431-85619803，E-mail：lipy@jlu.edu.cn ） E-mail:lipy@jlu.edu.cn
  • 作者简介:金 鑫（1986-），男，吉林省敦化市人，医师，医学硕士，主要从事肿瘤治疗方面的研究。
  • 基金资助:

     吉林省科技厅重大项目资助课题（20096039）

Protective effect of pseudo-ginsenoside GQ on doxorubicin-induced acute myocardial injury in rats

JIN Xin1,SHEN Wei-zhang1,JIN Li-fang1,JIA Jiao-yuan1,LI Xiao-feng1,WANG Xiu-li1,DI Xin*,ZHANG Hong-juan*,LI Ping-ya2   

  • Received:2013-11-02 Published:2013-12-12

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摘要：

目的：构建阿霉素(DOX)致大鼠急性心肌损伤模型，探讨伪人参皂苷GQ(PGQ)对大鼠急性心肌损伤的保护作用。方法：通过股静脉注射DOX建立大鼠急性心肌损伤模型，将30只雄性Wistar大鼠随机分为对照组、DOX组（18 mg/kg）、DOX+右丙亚胺(DZR)组（180 mg/kg）和DOX+低、中、高剂量PGQ组（分别为3、6和12 mg/kg/d）(n=5)。观察各组大鼠的一般状态，计算各组大鼠心脏/体质量（HW/BW）比值，检测各组大鼠心功能指标和血清肌钙蛋白I（TnI）水平，观察各组大鼠心肌组织形态学和超微结构改变。结果：与对照组比较，DOX组大鼠BW总下降值明显升高（P<0.05），HW/BW比值明显增高（P<0.05），心率（HR）、左心室收缩压（LVSP）和左心室内压力上升/下降最大速率（±dp/dtmax）明显降低（P<0.05），TnI水平明显增高（P<0.05），并见心肌组织形态学及超微结构损伤。与DOX组比较，DOX+DZR组和DOX+中剂量PGQ组大鼠HR、LVSP、±dp/dtmax明显升高（P<0.05），TnI水平明显减低（P<0.05），HW/BW比值明显减低（P<0.05），大鼠心肌组织形态学及超微结构改变明显好转。与DOX+DZR组比较，DOX+中剂量PGQ组大鼠BW总下降值明显减少（P<0.05），且对心肌细胞的超微结构保护作用更显著。结论：PGQ能够拮抗DOX所致的心肌损伤，具有心肌保护作用。

关键词: 伪人参皂苷GQ, 阿霉素, 心肌损伤, 保护作用

Abstract:

Abstract:Objective To establish doxorubicin　(DOX)-induced myocardial injury rat model and to explore the protective effect of the pseudo-ginsenoside GQ (PGQ) on DOX-induced myocardial injury in rats. Methods The  acute myocardial injury  model was established through the femoral vein injection of doxorubicin,and 30 Wistar male rats were randomly divided into  control group(n=5),DOX group(18 mg?kg-1,n=5),DOX+dexrazoxane(DZR) group(180 mg/kg,n=5);DOX+low,medium,high doses of  PGQ groups(3,6,12 mg/kg/d,n=5). The general state,the heart weight/body weight(HW/BW) ratio, the  cardiac function indexes,the levels of  serum troponin(TnI) of the rats in various groups were detected and the  morphological and ultrastructural changes of myocardium tissue of the rats were observed. Results Compared with  control group,the total decline value of BW of the rats in DOX group was significantly increased(P<0.05);HW/BW ratio was  significantly increased(P<0.05); heart rate(HR),left ventricular systolic(LVSP) and ±dp/dtmax were  significantly decreased(P<0.05),and the level of serum TnI was significantly increased(P<0.05).The morphological and ultrastructural injuries of the myocardium tissue were observed. Compared with DOX group,the HR,LVSP,±dp/dtmax  of the rats in DOX+DZR group and DOX+medium dose of PGQ group were significantly increased(P<0.05),and the levels of serum TnI were significantly decreased(P<0.05),and HW/BW ratios were  significantly decreased(P<0.05).The morphological and ultrastructural injuries were significantly improved compared with DOX group. Compared with DOX+DZR group,the total decline value of BW of the  rats in  DOX+medium dose of PGQ group was significantly decreased(P<0.05),and the  ultrastructural injury was significantly improved.Conclusion  PGQ has the protective effect of myocardial injury against DOX-induced myocardial injury.

Key words: pseudo-ginsenoside GQ, doxorubicin, myocardial injury, protective effect

中图分类号: 

  • R730.5
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