吉林大学学报(医学版)

• 基础研究 • 上一篇    下一篇

抗抑郁药奥氮平对大鼠海马神经细胞凋亡和细胞周期的影响

刘颖1,2, 张萱3, 刘纯岩4,倪文杰1,毛洪涛1,王珍琦1   

  1. 1.吉林大学公共卫生学院 卫生部放射生物学重点实验室,吉林 长春 130021;2.吉林省妇幼保健院中心实验室,吉林 长春130061;3.吉林大学第二医院科教科,吉林 长春130041;4.吉林大学第二医院放射线科,吉林 长春130041
  • 收稿日期:2013-04-08 出版日期:2013-11-28 发布日期:2013-11-28
  • 通讯作者: 王珍琦(Tel:0431-85619443,E-mail:wangzq@jlu.edu.cn) E-mail:wangzq@jlu.edu.cn
  • 作者简介:刘 颖(1973-),女,吉林省长春市人,副教授,医学硕士,主要从事抗精神病药神经保护作用的研究。
  • 基金资助:

    olanzapine;cell cycle;apoptosis;neuroprotection;glutamate;neuron

Effects of olanzapine on apoptosis and cell cycle of  hippocampus neurons  in rats

LIU Ying1,2,ZHANG Xuan3,LIU Chun-yan4,NI Wen-jie1,MAO Hong-tao1,WANG Zhen-qi1   

  1. 1.Key Laboratory of Radiobiology,Ministry of Health,School of Public Health,Jilin University,Changchun 130021,China;2. Central Laboratory,Jilin Women and Children Health Hospital,Changchun 130061,China;3. Department of Science and Education,Second Hospital,Jilin University,Changchun 130041,China;4. Department of Radiology,Second Hospital,Jilin University,Changchun 130041,China
  • Received:2013-04-08 Online:2013-11-28 Published:2013-11-28

摘要:

目的:探讨抗抑郁药物奥氮平对谷氨酸(Glu)损伤的大鼠海马神经元凋亡、坏死和细胞周期的影响,阐明奥氮平神经保护作用的机制。方法:采用体外原代培养的大鼠海马神经元,复制Glu损伤模型,细胞分为正常对照组、Glu损伤组和奥氮平+Glu损伤组,在体外通过流式细胞术测定各组神经元细胞凋亡率、坏死率和细胞周期的改变。结果:与正常对照组比较,其他组细胞凋亡率增加 (P<0.01);与Glu损伤组比较,10.0 μmol/L奥氮平+Glu损伤组细胞凋亡率降低(P<0.01 )。与正常对照组比较,Glu损伤组神经元坏死率升高2.9倍 (P<0.05);与Glu损伤组比较,100.0和200.0 μmol/L奥氮平+Glu损伤组神经元坏死率降低(P<0.05或P<0.01)。与正常对照组比较,Glu损伤组G0/G1期细胞百分数增加 (P<0.01),S期细胞百分数下降(P<0.01),G2 + M期变化不明显;而与Glu损伤组比较,
50.0和100.0 μmol?L-1奥氮平+Glu损伤组G0/G1期细胞比例下降为Glu损伤组的79.9%和62.6% (P<0.05或P<0.01);S期细胞比例上升为Glu损伤组的2.5和3.8倍 (P<0.05或P<0.01)。结论:奥氮平能够抵抗Glu诱导的神经元凋亡和坏死,改变细胞周期进程。

关键词: 奥氮平, 细胞周期, 细胞凋亡, 神经保护作用, 谷氨酸, 神经元

Abstract:

Abstract:Objective To investigate the effects of antidepressant olanzapine on the apoptosis,death  and cell cycle of  hippocampus neurons of the rats injured by glutamate (Glu),and to  illuminate the mechanism of neuroprotive effect of olanzapine.Methods The primary cultured hippocampal neurons were injured with Glu to set up Glu-injured models.The neurons  were divided into normal  control group,Glu injury group and olanzapine+Glu injury group.The changes of   apoptotic rate,death rate and cell cycle of neurons were detected by FCM,respectively.Results Compared with normal control group,the  apoptotic rate of neurons in the other  groups were increased (P<0.01);compared with Glu injury group,the apoptotic rate  of cells in 10.0 μmol/L olanzapine+Glu injury groupwas decreased(P<0.01).Compared with normal control group,the death rate of neurons was up to 2.9 times of that in Glu injury group(P<0.05);
compared with Glu injury group,the death rates of neurons in 100.0 and 200.0 μmol/L olanzapine+Glu groups were decreased (P<0.05 or P<0.01).Compored with normal control group,the  percentage of neurons in G0/G1 phase in Glu injury group was increased (P<0.01),and the  percentage of neurons in S phase was decreased (P<0.01),but the  percentage of neurons in G2+M phase didn’t change significantly;compared with Glu injury group,the
  percentages of   G0/G1 phase  in 50.0 and 100.0 μmol/L olanzapine+Glu injury groups were decreased to 79.9% and 62.6% of that in Glu injury group
(P<0.05 or P<0.01).and the  percentage of neurons in S phase  were up to 2.5 an 3.8 times of that in Glu injury group (P<0.05 or P<0.01).Conclusion
Olanzapine can prevent the apoptosis and death of hippocampal neurons induced by Glu,and change the cell cycle.

中图分类号: 

  • R737.33