关键词: 内皮, 血管, 内皮生长因子, 肿瘤转移, 新生血管化, 病理

Abstract: Objective To investigate the effects of HGF/SF and VEGF on vascular endothelial cells proliferation and capillary sprouts formation. Methods Endothelial cells proliferation test and capillary sprouts formation test were used to evaluate the effects of HGF/SF and VEGF on human umbilical artery endothelial cells (HUAECs) proliferation and capillary sprouts formation. ELISA was used to measure the contents of HGF/SF and VEGF in the media. The rat cornea neovascularization was used to evaluate the effects of HGF/SF and VEGF on angiogenesis in vivo. Results HGF/SF and VEGF had obvious effects on angiogenesis and endothelial cells proliferation and capillary sprouts formation. The VEGF was found in HGF/SF media. Conclusion The combination use of HGF/SF and VEGF has cooperative effects on angiogenesis in tumor, HGF/SF may have the effect to stimulate vascular endothelial cells to produce VEGF.

Key words: endothelium, vascular, endothelial growth factors, neoplasm metastasis, neovascularization, pathologic