吉林大学学报(医学版)

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结直肠癌组织中树突状细胞标志物CD1a和CD83的表达及意义

魏静波1,刘 辉2,谢朝辉3,曲银娥1   

  1. 1.  河北联合大学组织胚胎学系,河北 唐山 063000;2. 河北联合大学校长办公室,
    河北 唐山 063000;3. 河北省承德市肿瘤医院,河北 承德 067000
  • 收稿日期:2013-11-06 出版日期:2014-07-28 发布日期:2015-01-18
  • 通讯作者: 曲银娥(Tel: 0315-3725754,E-mail: linlin0852@sina.com) E-mail:linlin0852@sina.com
  • 作者简介:魏静波(1978-),女,内蒙古自治区赤峰市人,讲师,医学硕士,主要从事 生殖免疫和肿瘤免疫的研究。
  • 基金资助:

    河北省科技厅科研基金资助课题(12277747);河北省唐山市科技局科研基金
    资助课题(12150222B-16)

Expressions of dendritic cells markers CD1a and CD83
in colorectal cancer tissue and  significance

WEI Jing-bo1,LIU Hui2,XIE Zhao-hui3,QU Yin-e1   

  1. 1. Department of Histology and Embryology,Hebei United University,Tangshan 063000,China;
    2. Headmaster’s Office,Hebei United University,Tangshan 063000,China;3. Chengde Tumor Hospital,Chengde 067000,China
  • Received:2013-11-06 Online:2014-07-28 Published:2015-01-18

摘要:

目的:探讨结直肠癌组织中不同分化阶段树突状细胞(DCs)形态、数量和表型的差异,阐明DCs在结直肠癌发生和发展中的作用。方法:收集结直肠癌标本20例(结直肠癌组),同时选取癌旁正常组织(距癌>5 cm)20例作为正常对照组,应用免疫组织化学法检测不同分化阶段DCs的分布、形态和数量。应用Western blotting法检测DCs表面标志物CD1a和CD83的表达。结果:免疫组织化学法,2组DCs均分布于腺体和血管间;2组CD1a+未成熟型DCs(imDCs)均呈圆形和卵圆形,树突状突起依稀可见,结直肠癌组CD1a+imDCs的数量较正常对照组明显增多(P<0.01);2组CD83+成熟型DCs(mDCs)形态均不规则,有多个突起,呈分枝状,结直肠癌组CD83+mDCs的数量较正常对照组明显减少(P<0.01)。Western blotting法,与正常对照组比较,结直肠癌组CD1a蛋白表达水平明显升高(P<0.01),而CD83蛋白表达
水平明显降低(P<0.01)。结论:CD1a+imDCs在结直肠癌的发生发展中可能具有促进作用,而CD83+mDCs在结直肠癌的发生发展中可能具有抑制作用。
  

关键词:  , 树突状细胞;结直肠肿瘤;免疫应答;免疫耐受

Abstract:

Abstract:Objective To investigate the differences in the structures,numbers and phenotypes of the dendritic cells (DCs) at different stages in colorectal cancer
(CRC) tissue, and to explore the effect of DCs in the occurrence and development of CRC.Methods 20 cases of CRC tissue were chosen as CRC
 group,other 20 cases of corresponding para-cancer tissue were chosen as control group.Immunohistochemical staining was used to detect the distributio
n,the structures,the number of positive cells at different stages;Western blotting was used to detect the expressions of CD1a and CD83.
Results The results of immunohistochemical staining showed that DCs scattered among glands or vessels in both groups;the CD1a+imature DCs (imDCs) were round and oval,dendritic processes were faintly visible in both groups,and the number of CD1a+imDCs in experimental group was significantly higher than that in control group (P<0.01).The CD83+ mature DCs (mDCs) were irregular,branched in both groups,and the number of CD83+mDCs in CRC  group was significantly lower than that in control group (P<0.01).The results of Western blotting method showed that the expression of CD1a protein was higher and the expression of CD83 protein was lower in CRC  group than  control group(P<0.01).Conclusion CD1a+imDCs may play important roles in promoting the occurrence and development of CRC;CD83+mDCs may inhibit the
 occurrence and development of CRC.

Key words: dendritic cells, colorectal neoplasms, immune response, immune tolerance

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