热休克蛋白65-MUC1融合蛋白对小鼠干扰素γ产生细胞的诱生作用

doi:国家“973”资助课题

热休克蛋白65-MUC1融合蛋白对小鼠干扰素γ产生细胞的诱生作用

李大鹏¹,王莉²,卫红飞¹,张培因¹,吴秀丽¹,万敏¹,王燕媚¹,王爱丽¹,杨世杰¹ ,王丽颖^1*

1. 吉林大学基础医学院分子生物学教研室, 吉林长春130021；2. 吉林大学基础医学院免疫学教研室, 吉林长春130021

收稿日期:2003-12-05 修回日期:1900-01-01 出版日期:2004-05-28 发布日期:2004-05-28
通讯作者: 王丽颖

Priming of mice lymphocytes for secretion of IFNγby heat shock protein-MUC1 fusion protein

LI Da-peng¹, WANG Li², WEI Hong-fei¹, ZHANG Pei-yin¹, WU Xiu-li¹, WAN Min¹,WANG Yan-mei¹,WANG Ai-li¹,YANG Shi-jie¹,WANG Li-ying^1*

1. Department of Molecular Biology, School of Basic Medical Sciences,Jilin University, Changchun 130021, China;2. Department of Immunology, School of Basic Medical Sciences,Jilin University，Changchun 130021, China

Received:2003-12-05 Revised:1900-01-01 Online:2004-05-28 Published:2004-05-28
Contact: WANG Li-ying

摘要/Abstract

摘要： 目的：研究热休克蛋白65-MUC1（HSP65-MUC1）融合蛋白对小鼠免疫细胞的激活作用。方法：用HSP65-MUC1融合蛋白免疫C57BL/6小鼠3次后，取脾脏和淋巴结，分离淋巴细胞，体外用HSP65-MUC1融合蛋白、MUC1肽或非特异性刺激剂如CpG ODN和ConA进行刺激，采用酶联免疫斑点法（enzyme linked immunospot, ELISPOT）检测免疫细胞分泌干扰素γ的情况。结果：HSP65-MUC1融合蛋白免疫后小鼠的淋巴细胞在特异性或非特异性抗原刺激下所分泌的干扰素γ比PBS组明显增多。结论：HSP65-MUC1融合蛋白对小鼠干扰素γ产生细胞有很强的诱生作用。

关键词: 干扰素Ⅱ型, 诱生作用

Abstract: Objective To study the role of heat shock protein65-MUC1 (HSP65-MUC1) fusion protein in the priming of IFNγ producing cells from immunized mice by detecting their IFNγ secretion. Methods The lymphocytes from spleen and lymph nodes of C57BL/6 mice immunized three times with HSP65-MUC1 fusion protein were prepared and then stimulated by HSP65-MUC1 fusion protein, MUC1 peptides or nonspecific stimulator such as CpG ODN and Con A in vitro were used to evaluate their IFNγ secretion through enzyme linked immunospot technique. Results The lymphocytes from the mice immunized by HSP65-MUC1 fusion protein produced much more IFNγ compared with those from control mice in the presence of specific or nonspecific stimulators. Conclusion HSP65-MUC1 fusion protein plays an important role in the priming of mice lymphocytes for the secretion of IFNγ.

Key words: interferon type Ⅱ, induction role

中图分类号:

李大鹏,王莉,卫红飞,张培因,吴秀丽,万敏,王燕媚,王爱丽,杨世杰 ,王丽颖. 热休克蛋白65-MUC1融合蛋白对小鼠干扰素γ产生细胞的诱生作用[J]. J4, 2004, 30(3): 405-408.

LI Da-peng, WANG Li, WEI Hong-fei, ZHANG Pei-yin, WU Xiu-li, WAN Min,WANG Yan-mei,WANG Ai-li,YANG Shi-jie,WANG Li-ying. Priming of mice lymphocytes for secretion of IFNγby heat shock protein-MUC1 fusion protein[J]. J4, 2004, 30(3): 405-408.

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