urantide对大鼠血管平滑肌细胞增殖的影响

J4 ›› 2011, Vol. 37 ›› Issue (6): 1079-1082.

urantide对大鼠血管平滑肌细胞增殖的影响

赵娟¹, 王红², 于全鑫¹, 石艳³, 李相军³, 任立群³

(1.承德医学院病理生理学教研室|河北承德 067000；2.吉林职工医科大学实训中心|吉林　吉林　132011；3.吉林大学药学院实验药理与毒理学教研室|吉林长春 130021)

收稿日期:2011-07-01 出版日期:2011-11-28 发布日期:2013-04-11
通讯作者: 任立群 E-mail:（Tel：0431-85619702，E-mail：renlq@jlu.edn.cn）
作者简介:赵娟（1981-）|女|内蒙古自治区赤峰市人|医学博士|主要从事病理医学研究。
基金资助:
承德医学院博士基金资助课题（201102）

Effect of urantide on proliferation of vascular smooth muscle cells in rats

ZHAO Juan¹|WANG Hong²,YU Quan-xin¹|SHI Yan³|LI Xiang-jun³|REN Li-qun³

(1. |Department of Pathophysiology,Chengde Medical College,Chengde 067000,China;2.Training Center,Jilin Staff University of Medical Sciences,Jilin 132011,China;3. Department of Experimental Pharmacology and Toxicology,School of Pharmacy,Jilin University,Changchun 130021,China)

Received:2011-07-01 Online:2011-11-28 Published:2013-04-11

摘要/Abstract

摘要：

目的：探讨在尾加压素Ⅱ（UⅡ）受体拮抗剂urantide干预下，UⅡ在大鼠血管平滑肌细胞（VSMC）中的表达，阐明其可能的作用机制。方法：采用贴块法对大鼠胸主动脉血管平滑肌细胞进行体外培养，实验分为正常对照组（C组）、UⅡ组（M组）、阳性药对照组（Flu组）及urantide干预组(浓度分别为1×10^-10、1×10^-9、1×10^-8、1×10-⁷和1×10^-6 mol·L^-1)，采用MTT法检测VSMC增殖活性，采用流式细胞术测定VSMC增殖指数（PI）及S期细胞分数（SPF）。结果：与正常对照组比较， UⅡ组在各时间点VSMC增殖活性均增加（P<0.01）；PI及SPF明显增加（P<0.01）；与UⅡ组比较，1×10^-10～10^-6 mol?L-1urantide组VSMC增殖活性明显降低（P<0.05或P<0.01），PI及SPF均明显减少（P<0.05或P<0.01），其中以1×10^-6 mol·L^-1urantide作用最明显。结论： urantide可阻断UⅡ对动脉粥样硬化（AS）主要病变细胞VSMC的促丝裂作用，本研究为临床应用urantide治疗AS提供了新的视角和实验依据。

关键词: 尾加压素Ⅱ；urantide；血管平滑肌细胞；动脉粥样硬化

Abstract:

Abstract:Objective
To investigate the expressions of both urotensin Ⅱ(UⅡ) and its receptor GPR14 in rat vascular smooth muscle cells (VSMC) under the intervention of urantide (UⅡ receptor antagonist),and clarify its possible mechanism.Methods The VSMC from rat thoracic aorta were cultivated in vitro with tissue explant method,and divided into normal control group (C group),UⅡ group (M group),positive control group (Flu group) and five intervention groups (10^-10,10^-9,10^-8,10^-7 and 10-6 mol·L^-1 urantide).The proliferation index(PI) and S-phase cell fraction(SPF) of in vitro cultured VSMC were detected with MTT method and flow cytometry.Results Compared with C group,the VSMC proliferation was increased at each time point(P<0.01),and PI and SPF were also increased significantly (P<0.01);compared with M group, the VSMC proliferation in 10^-10－10^-6 mol·L^-1 urantide groups were significantly decreased(P<0.05 or P<0.01)，and PI and SPF were significantly reduced (P<0.05 or P<0.01),among which 10-6 mol·L^-1 urautide group had the most significant effect. Conclusion Urantide can block the mitogenic effect of UⅡ on atherosclerosis(AS) of the major disease-promoting cells VSMC,providing a new perspective and experimental basis for clinical application of urantide to treat AS.

Key words: urotensin Ⅱ;urantide;vascular smooth muscle cells;atherosclerosis

中图分类号:

R363

赵娟, 王红, 于全鑫, 石艳, 李相军, 任立群. urantide对大鼠血管平滑肌细胞增殖的影响[J]. J4, 2011, 37(6): 1079-1082.

ZHAO Juan|WANG Hong,YU Quan-xin|SHI Yan|LI Xiang-jun|REN Li-qun . Effect of urantide on proliferation of vascular smooth muscle cells in rats[J]. J4, 2011, 37(6): 1079-1082.

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