吉林大学学报(医学版)

• 基础研究 • 上一篇    下一篇

不同下颌前伸力对髁突软骨细胞中Sox9和Ⅱ型胶原表达的影响及其意义

刘莹1,刘艳艳2,王硕1,袁洋1,刘畅1   

  1. 1.吉林大学口腔医院正畸科,吉林  长春  130021;2.吉林大学南岭校区医院口腔科,吉林 长春 130022
  • 收稿日期:2013-06-19 出版日期:2014-01-28 发布日期:2014-01-25
  • 通讯作者: 刘 畅 (Tel:0431-88796023,E-mail:lcwztt@gmail.com) E-mail:lcwztt@gmail.com
  • 作者简介:刘 莹(1986-),女,吉林省蛟河市人,医学硕士,主要从事口腔正畸基础与临床研究。
  • 基金资助:

    吉林省卫生厅科研基金资助课题(2008Z010);吉林大学基本科研业务费-科学前沿与交叉学科创新项目资助课题(200903225)

Effects  of different modes of mandibular advancement on expressions of Sox9 and collagen Ⅱ in condylar chondrocytes and their signific

LIU Ying1,LIU Yan-yan2,WANG Shuo1,YUAN Yang1,LIU Chang1   

  1. 1. Department of Orthodontics,Stomatology Hospital,Jilin University,Changchun 130021,China;2. Department of Stomatology,Hospital of Nanling Campus,Jilin   University,Changchun 130022,China
  • Received:2013-06-19 Online:2014-01-28 Published:2014-01-25

摘要:

目的:探讨施加不同作用方式的下颌前伸力后大鼠髁突软骨细胞中Sox9及Ⅱ型胶原表达水平,阐明不同作用方式的下颌前伸力对其表达及软骨形成的影响。方法:将28只5周龄雄性Wistar大鼠按下颌前伸力的不同作用方式随机分为动态组、静态组、功能组和对照组(n=7)。各组大鼠按照设定的相关参数接受不同的下颌前伸力刺激。2周后实验结束时取颞下颌关节标本,行免疫组织化学染色,对髁突软骨细胞中Sox9和Ⅱ型胶原的表达进行半定量分析。结果:对照组、动态组和功能组大鼠髁突软骨细胞中Sox9阳性表达细胞数显著高于静态组(P<0.01),动态组和对照组大鼠髁突Sox9阳性表达面积显著高于静态组和功能组(P<0.01),功能组Ⅱ型胶原阳性表达面积显著高于其他3组(P<0.01),静态组Ⅱ型胶原阳性表达面积显著高于对照组(P<0.05)。结论:下颌前伸力的作用方式及作用时间是调控髁突软骨细胞中Sox9及Ⅱ型胶原表达水平的关键因素。

关键词: 下颌前伸力, Sox9, Ⅱ型胶原, 软骨内成骨

Abstract:

Abstract:Objective To study the expression levels of Sox9 and collagen Ⅱ in condylar chondrocytes after treated with different modes of mandibular advancement in rats,and to clarify the effects of different modes of mandibular advancement on the expressions of Sox9 and collagen Ⅱ as well as formation of cartilage in condyle.Methods  Twenty-eight male 35-day-old Wistar rats were randomly divided into dynamic,static,functional and control groups(n=7),and received different modes of mandibular advancement according to their groups.2 weeks  after the experiment,temporomandibular joint sections were prepared for immunohistochemical staining,and the expression levels of Sox9 and collagen Ⅱ in condylar chondrocytes were semi-quantitatively evaluated.Results The number of Sox9 positive cells in control group,dynamic group and functional group was significantly higher than that in static group(P<0.01),the Sox9 positive expression areas in dynamic group and control group were statistically larger than those in static group and functional group(P<0.01),the positive expression area of collagen Ⅱ in functional group was significantly higher than those in the other three groups(P<0.01),and the positive  expression area of collagen Ⅱ in static group was higher than that in control group(P<0.05).Conclusion The modes and duration of mandibular advancement play a key role in regulating the expressions of Sox9 and collagen Ⅱ in condylar chondrocytes.

Key words: mandibular advancement, Sox9, collagen Ⅱ, endochondral ossification

中图分类号: 

  • R783