吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (04): 699-703.doi: 10.13481/j.1671-587x.20160413

• 基础研究 • 上一篇    下一篇

激活素A通过下丘脑室旁核调节动脉压的作用及其机制

范钰奇1, 王明华1, 谢东雪2, 崔雪玲2, 葛敬岩1   

  1. 1. 吉林大学基础医学院生理学系, 吉林 长春 130021;
    2. 吉林大学基础医学院遗传学系, 吉林 长春 130021
  • 收稿日期:2016-01-25 发布日期:2016-07-20
  • 通讯作者: 葛敬岩,副教授,硕士研究生导师(Tel:0431-85619472,E-mail:gejy@jlu.edu.cn) E-mail:gejy@jlu.edu.cn
  • 作者简介:范钰奇(1990-),女,河南省漯河市人,医学硕士,主要从事神经生理学方面的研究。
  • 基金资助:

    吉林省科技厅国际合作基金资助课题(20130413001GH)

Effect of activin A on regulation of arterial blood pressure by hypothalamic paraventricular nucleus and its mechanism

FAN Yuqi1, WANG Minghua1, XIE Dongxue2, CUI Xueling2, CUI Xueling1   

  1. 1. Department of Physiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China;
    2. Department of Genetics, School of Basic Medical Sciences, Jilin University, Changchun 130021, China
  • Received:2016-01-25 Published:2016-07-20

摘要:

目的:探讨激活素A在WKY大鼠下丘脑室旁核(PVN)中的表达及其对动脉压的影响,阐明激活素A通过PVN调节动脉压的作用机制。方法:选用WKY大鼠,采用RT-PCR法检测激活素A、激活素Ⅱ型受体(ActRⅡA和ActRⅡB)和信号传导蛋白Smads mRNA在PVN中的表达情况,免疫组织化学染色检测ActRⅡA蛋白在PVN中的表达情况。PVN核团微量注射外源性激活素A,观察给药前后大鼠动脉压的变化。原代培养WKY大鼠PVN神经元,分为对照组和激活素A处理组,RT-PCR法检测PVN神经元中ActRⅡA、ActRⅡB和Smads mRNA表达水平。结果:WKY大鼠PVN中存在激活素A及其受体、Smad2和Smad3 mRNA表达,免疫组织化学染色检测,PVN神经元表达ActRⅡA蛋白。PVN微量注射血管紧张素Ⅱ(AngⅡ)或激活素A后,与给药前比较,大鼠平均动脉压明显升高(P < 0.05)。与对照组比较,激活素A处理组大鼠体外原代培养PVN神经元中ActRⅡA和Smad3 mRNA表达水平明显升高(P < 0.05)。结论:激活素A以自分泌/旁分泌形式通过PVN调控动脉压,其作用与ActRⅡA-Smad3信号传导途径有关。

关键词: 动脉血压, 下丘脑室旁核, 激活素A, Smad蛋白

Abstract:

Objective: To investigate the expression of activin A in paraventricular nucleus (PVN) of the WKY rats and its influence in arterial blood pressure,and to clarify the mechanism of activin A in the regulation of arterial blood pressure by PVN. Methods: The WKY rats were selected. The expressions of activin A,ActRⅡA,ActRⅡB,and Smads mRNA in PVN of the WKY rats were measured by RT-PCR. The expression of ActRⅡA protein in PVN was detected by immunohistochemical staining. The microinjection of exogenous activin A into PVN was used to observe the changes of arterial blood pressure. The primary cultured PVN neurons from the WKY rats were divided into control group and activin A group. The mRNA expression levels of ActRⅡA,ActRⅡB, and Smads in the PVN neurons were analyzed by RT-PCR. Results: Activin A,ActRⅡA,ActRⅡB,Smad2 and Smad3 mRNA were expressed in PVN of the WKY rats. The ActRⅡA protein expression in PVN was further confirmed by immunohistochemical staining. After microinjection of activin A or angiotensin Ⅱ (AgⅡ) into PVN,the mean arterial blood pressure was increased obviously compared with before treatment (P < 0.05). Moreover,compared with control group,the expression levels of ActRⅡA and Smad3 mRNA in primary cultured PVN neurons of the rats in vitro were significantly increased(P < 0.05). Conclusion: Activin A can regulate the arterial blood pressure in PVN in an autocrine or paracrine manner,which is related to ActRⅡA-Smad3 signal pathway.

Key words: arterial blood pressure, paraventricular nucleus, activin A, drosophia mothers against decapentaplegic protein

中图分类号: 

  • R-331