CHI3L1基因多态性和血清YKL-40水平与支气管哮喘发病的关联性

doi:10.13481/j.1671-587x.20140529

吉林大学学报(医学版)

CHI3L1基因多态性和血清YKL-40水平与支气管哮喘发病的关联性

陈敏^1，2，赖天文²，招轩娜²，吕莹莹²，高拯妮²，黄丹²，刘升明¹

1.暨南大学附属第一医院呼吸内科,广东广州 510630；2.广东医学院附属医院呼吸内
科,广东湛江 524001

收稿日期:2013-12-24 出版日期:2014-09-28 发布日期:2014-11-24
通讯作者: 刘升明(Tel：020-38688066，E-mail：tlsm@jnu.edu.cn) E-mail:tlsm@jnu.edu.cn
作者简介:陈敏(1976-)，男，广东省兴宁市人，副主任医师，医学硕士，主要从事支气管哮喘防治的研究。
基金资助:
广东省科技厅科技计划项目资助课题（2010B031600285）

Correlation between CHI3L1 polymorphism and serum YKL-40 and bronchial asthma 

CHEN Min^1,2,LAI Tian-wen²,ZHAO Xuan-na²,LYU Ying-ying²,GAO Zheng-ni²,HUANG Dan²

1. Department of Respiratory Medicine,First Affiliated Hospital,Jinan U
niversity,Guangzhou 510630,China;2. Department of Respiratory Medicine,Affiliated Hospital，Guangdong Medical C
ollege,Zhanjiang 524001,China

Received:2013-12-24 Online:2014-09-28 Published:2014-11-24

摘要/Abstract

摘要：

目的:观察几丁质酶3类1(CHI3L1)基因多态性与广东地区汉族人群哮喘发病的关联性，探讨采用其编码的几丁质酶样蛋白40（YKL-40）评估病情严重程度及监测病情的临床意义。方法：入选的251例研究对象分为哮喘组(150例)和正常对照组(101例)。采用Massarray法检测CHI3L1基因单核苷酸多态性(SNP)，同时检测哮喘组和对照组研究对象血清YKL-40水平、外周血嗜酸性粒细胞百分比（Eos%）、总IgE和肺功能，同时将哮喘组分为急性加重期和稳定期2个
亚组，比较患者血清YKL-40水平的差异。结果：CHI3L1基因位点rs3806448 A/G等位基因在哮喘组和对照组中分布比较差异有统计学意义（P<0.01），哮喘组患者rs3806448等位基因A频数明显高于正常对照组（OR=1.93,95%CI=1.30~2.62,χ2=11.6,P<0.01）；rs3806448基因型为AA患者
血清YKL-40水平、总IgE和Eos%高于基因型为GG或AG患者（P<0.05）；而rs3806448基因型为AA患者一秒用力呼气容积（FEV1）占预计值百分比(FEV1%)低于基因型为GG或AG患者（P<0.05）；哮喘组患者血清YKL-40水平明显高于正常对照组（P<0.01）,哮喘急性加重组患者血清YKL-40水平高于病情稳定组
和正常对照组（P<0.01）；哮喘组患者血清YKL-40水平与外周血EOS%及总IgE水平呈正相关关系（rEos=0.348，rIgE =0.437，P<0.01）,与肺功能呈负相关关系（r=－0.745，P<0.01）。结论：CHI3L1-rs3806448多态性与广东地区汉族人群的哮喘发病有关联，提示血清YKL-40可能是评价哮喘
患者病情严重程度及监测病情变化的一个新生物标志物。

关键词: 支气管哮喘, 单核甘酸多态性, 几丁质酶3样蛋白1

Abstract:

Objective To investigate the association between CHI3L1 polymorphism and asthma in the Han population of Guangdong,and to explore the clinical values of chitinase-like protein YKL-40 in evaluating disease severity and monitoring the progression of the disease.Methods Total 251 objects were divided into asthma group(n=150) and control group(n=101).The single nucleotide polymorphism (SNP) at CHI3L1 gene was detected with Massarray method.The serum YKL-40 levels,total IgE levels,peripheral blood eosinophil percentage(Eos%) of the objects in asthma and control groups were detected.The asthma patients were divied into asthma exacergation group and stable group, and the serum YKL-40 levels were compared.Results The distribution
of A/G at rs3806448 of CHI3L1 had significant difference between asthma and control groups(P<0.01),and the frequency of A allele at rs3806448 of the pati
ents in asthma group was significantly higher than that in control group(OR= 1.93,95% CI=1.30－2.62,χ2=11.6,P<0.01).The serum YKL-40 levels，total IgE and Eos% of the patients with AA at rs3806448 were higher than those of the patients with GG or AG at rs3806448 (P<0.05);the FEV1% of the patients with AA was lower than those of the patients with GG or AG at rs3806448(P<0.01).The serum YKL-40 level of the patients in asthma group was significantly highe
r than that in normal control group(P<0.01),and the serum YKL-40 level of the patients in asthma exacergation group was higher than those in asthma stable group and normal control group(P<0.01).The serum YKL-40 level of the patients in asthma group was positively correlated with the peripheral blood Eos% and total IgE level(rEos=0.348，rIgE=0.437，P<0.01),and was negatively correlated with the lung function(r=－0.745，P<0.01). Conclusion
The polymorphism of CHI3L1-rs3806448 may correlate with asthma of Han population in Guangdong area.The serum YKL-40 may be a new biomarker to
evaluate asthma disease severity and helpful for monitoring illness stage.

Key words: bronchial asthma, single nucleotide polymorphism, chitotriosidase 3-like 1

中图分类号:

R394

陈敏，赖天文，招轩娜，吕莹莹，高拯妮，黄丹，刘升明. CHI3L1基因多态性和血清YKL-40水平与支气管哮喘发病的关联性[J]. 吉林大学学报(医学版), 2014, 40(05): 1058-1063.

CHEN Min,LAI Tian-wen,ZHAO Xuan-na,LYU Ying-ying,GAO Zheng-ni,HUANG Dan. Correlation between CHI3L1 polymorphism and serum YKL-40 and bronchial asthma [J]. Journal of Jilin University Medicine Edition, 2014, 40(05): 1058-1063.

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CHI3L1基因多态性和血清YKL-40水平与支气管哮喘发病的关联性

Correlation between CHI3L1 polymorphism and serum YKL-40 and bronchial asthma 

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