吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (01): 101-105.doi: 10.13481/j.1671-587x.20170120

• 临床研究 • 上一篇    下一篇

支气管哮喘患儿血清骨膜蛋白水平变化及其意义

李艳凤1, 王洪波1, 高阳1, 郑雨轩2, 刘丽1   

  1. 1. 吉林大学第一医院小儿呼吸一科, 吉林 长春 130021;
    2. 吉林大学第一医院转化医学研究院, 吉林 长春 130061
  • 收稿日期:2016-06-23 出版日期:2017-01-28 发布日期:2017-02-08
  • 通讯作者: 刘丽,副教授,主任医师,硕士研究生导师(Tel:0431-88782975,E-mail:liuli0205@163.com) E-mail:liuli0205@163.com
  • 作者简介:李艳凤(1989-),女,山东省聊城市人,在读医学硕士,主要从事小儿支气管哮喘的基础和临床方面的研究。
  • 基金资助:

    国家卫计委医药卫生科技发展研究中心科研项目资助课题(W2015EAE009)

Change of serum periostinlevel in children with bronchial asthma and significance

LI Yanfeng1, WANG Hongbo1, GAO Yang1, ZHENG Yuxuan2, LIU Li1   

  1. 1. First Department of Pediatric Respiratory Medicine, First Hospital, Jilin University, Changchun 130021, China;
    2. Institute of Translational Medicine, First Hospital, Jilin University, Changchun 130061, China
  • Received:2016-06-23 Online:2017-01-28 Published:2017-02-08

摘要:

目的:探讨支气管哮喘患儿血清中骨膜蛋白水平的变化,阐明其在哮喘中的作用。方法:随机选取支气管哮喘患儿65例作为观察组,健康儿童50人作为对照组,采用酶联免疫吸附测定(ELISA)法检测受试者外周血中骨膜蛋白、IgE和嗜酸细胞阳离子蛋白(ECP)水平及嗜酸性粒细胞计数,并检测呼出气一氧化氮(FeNO)、一秒用力呼气容积占预计值百分比(FEV1% pred)、用力肺活量(FVC)和FEV1/FVC比值。结果:与对照组比较,观察组患儿外周血中骨膜蛋白、IgE和ECP水平、嗜酸性粒细胞计数和FeNO均明显升高(P<0.05),FEV1% pred和FEV1/FVC比值均明显降低(P<0.05);采用血清骨膜蛋白水平诊断哮喘,受试者工作特征曲线(ROC曲线)下面积(AUC)为0.668(P=0.02),骨膜蛋白水平诊断哮喘界点为80.89 μg·L-1(95% CI:0.570~0.766),灵敏度为29.23%,特异度为92.00%;骨膜蛋白水平与嗜酸性粒细胞计数和FeNO呈正相关关系(r=0.519,P=0.019;r=0.602,P=0.008)。结论:哮喘患儿血清中骨膜蛋白水平升高,骨膜蛋白可以作为初步排查儿童哮喘的指标,抑制Th2介导的炎性反应过程是治疗哮喘的靶点。

关键词: 骨膜蛋白, 气道高反应性, 支气管哮喘, 酶联免疫吸附测定

Abstract:

Objective: To explore the change of serum periostin level in peripheral blood of the children with bronchial asthma,and to clarify its role in asthma.Methods: Sixty-five children with bronchial asthma were randomly chosen as observation group,and 50 healthy children were also randomly chosen as control group.The levels of periostin,IgE,eosinophil cationic protein(ECP) and the number of eosinophils in peripheral blood of all subjects were detected by enzyme-linked immunosorbent assay (ELISA). The fractional exhaled nitric oxide (FeNO) levels and pulmonary function test indexes including forced expiratory volume in one second/predicted value ratio (FEV1% pred),forced vital capacity (FVC) and FEV1/FVC ratio were also detected.Results: Compared with control group,the serum periostin,IgE,and ECP levels,the number of eosinophils and FeNO in observation group were significantly increased (P<0.05),while the FEV1%pred and FEV1/FVC ratio were significantly decreased (P<0.05).The area under ROC curve (AUC) was 0.668 (P=0.02) and the threshold for periostin to identify asthma was 80.89 μg·L-1 (95% CI:0.570-0.766) with the sensitivity of 29.23% and specificity of 92.00%. Periostin was positively correlated with the eosinophil count as well as FeNO (r=0.519, P=0.019;r=0.602,P=0.008).Conclusion: The periostin level in peripheral blood of the children with asthma is significantly increased and it can be used as a preliminary indicator to rule out asthma in the children.Inhibiting Th2-mediated inflammatory response is one target for the treatment of asthma.

Key words: bronchial asthma, airway hyperresponsiveness, enzyme-linked immunosorbent assay, periosteum protein

中图分类号: 

  • R725.6