关键词: ClC-3 , NF-κB , 免疫组织化学

Abstract: To investigate the expressions of ClC-3 and NF-κB in transitional cell carcinoma of the bladder(TCCB) specimen and their significances. MethodsThe expressions of ClC-3 and NF-κB were detected in 31 cases of TCCB and 6 cases of normal bladder tissue as control by immunohistochemical staining.Results In the tissues of TCCB, ClC-3 was mainly located in the cell membrane，the positive rate of ClC-3 was 74% (23 /31);the posivive rates of Ⅰ,Ⅱ and Ⅲ grades were 40%(4/10), 81%(9/11), and 100%(10/10), respectively. NF-κB was mainly distributed in the cytoplasm, the positive rate of NF-κB was 83% (26/31); the positive rates of Ⅰ, Ⅱ and Ⅲ grades were 60%(6/10), 90%(10/11) and 100%(10/10), respectively. Only one specimen showed weak positive staining, and negative staining of NF-κB was found in 6 cases of normal bladder tissues.The positive rates of ClC-3 and NF-κB in tissue of TCCB were higher than those in normal bladder tissue（P<0.01）.Conclusion ClC-3 and NF-κB may be related to the pathologic al grade and proliferation of TCCB.

Key words: ClC-3 , NF-κB , immunohistochemistry