SOX2对牙髓干细胞神经分化的促进作用及其意义

doi:10.13481/j.1671-587x.20160607

吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (06): 1076-1080.doi: 10.13481/j.1671-587x.20160607

SOX2对牙髓干细胞神经分化的促进作用及其意义

袁静¹, 陈耀宇², 赵艺帆², 刘小博², 刘朋飞², 赵雷¹

1. 暨南大学第二临床医学院深圳市人民医院麻醉科, 广东深圳 518020;
2. 吉林大学药学院再生医学系, 吉林长春 130021

收稿日期:2016-04-01 出版日期:2016-11-28 发布日期:2016-12-02
通讯作者: 赵雷,主任医师(Tel:0755-22948275,E-mail:zhaoleicx@126.com) E-mail:zhaoleicx@126.com
作者简介:袁静(1978-),女,广东省深圳市人,副主任医师,主要从事麻醉学基础和应用方面的研究。
基金资助:
吉林省科技厅科技发展计划项目资助课题（20120955）；广东省深圳市科技局知识创新计划资助课题（JCYJ20140416122812052）；广东省深圳市卫计委卫生计生系统科研项目资助课题（201401010）；吉林大学研究生创新基金资助课题（2015009）

Promotive effect of SOX2 on neural differentiation of dental pulp stem cells and its significance

YUAN Jing¹, CHEN Yaoyu², ZHAO Yifan², LIU Xiaobo², LIU Pengfei², ZHAO Lei¹

1. Department of Anesthesiology, Second Clinical Medical College, Jinan University, Shenzhen People's Hospital, Shenzhen 518020, China;
2. Department of Regenerative Medicine, School of Pharmacy, Jilin University, Changchun 130021, China

Received:2016-04-01 Online:2016-11-28 Published:2016-12-02

摘要/Abstract

摘要：

目的：探讨转录因子SOX2对牙髓干细胞（DPSCs）神经分化的影响，为研究DPSCs神经分化的机制提供依据。方法：从人源第三磨牙牙髓中分离DPSCs（DPSCs组），通过逆转录病毒感染方法构建过表达SOX2的DPSCs（DPSCs-SOX2组），以空白载体感染的DPSCs作为对照组（DPSCs-vector组），采用神经分化培养基诱导各组DPSCs分化为神经前体细胞（NPCs）。采用CCK-8法分析各组NPCs的增殖指数（PI）；采用qPCR和流式细胞术检测各组NPCs中Nestin和Pax6基因的表达水平；采用免疫荧光和流式细胞术分析各组NPCs在神经元分化方面的能力和分化后β3-Tubulin的表达效率。结果：通过神经分化培养基的定向诱导，正常DPSCs、DPSCs-vector和DPSCs-SOX2均可通过分化形成NPCs，但DPSCs-SOX2组NPCs的PI和NPCs中Nestin和Pax6表达水平明显高于DPSCs组和DPSCs-vector组（P<0.05）；DPSCs-SOX2组NPCs在神经元分化方面也具有一定的优势，分化后细胞的β3-Tubulin表达效率明显高于其他2组（P<0.05）。结论：SOX2对DPSCs的神经分化具有一定的促进作用。

关键词: SOX2, 神经分化, 神经前体细胞, 细胞增殖, 牙髓干细胞, 细胞治疗

Abstract:

Objective: To explore the effect of SOX2 on the neural differentiation of dental pulp stem cells (DPSCs), and to provide basis for studying the mechanism of neural differentiation of DPSCs.Methods: The DPSCs (DPSCs group) were isolated from human dental pulp of third molar, and the SOX2 over exression DPSCs (DPSCs-SOX2 group) were established via retrovirus infection. The empty vector-infected DPSCs (DPSCs-vector group) were regarded as control group. The DPSCs in various groups were induced into neural progenitor cells (NPCs) by neural differentiation medium. The proliferation index (PI) of NPCs in various groups was evaluated by CCK-8 method; the expression levels of Nestin and Pax6 gene in NPCs in various groups were detected by qPCR and FACS; the differentiation abilities of NPCs and the expression efficiencies of β3-Tubulin after differentiation in various groups were evaluated by immunofluorescence and FACS methods.Results: All of normal DPSCs, DPSCs-vector and DPSCs-SOX2 derived from NPCs could be differentiated into NPCs successfully through the induction of neural differentiation medium. Compared with DPSCs and DPSCs-vector groups, the PI of DPSCs in DPSCs-SOX2 group was increased (P<0.05), and the expression levels of Nestin and Pax6 in NPCs were increased (P<0.05). The expression efficiency of β3-Tubulin in differentiated NPCs in DPSCs-SOX2 group was higher than those in DPSCs and DPSCs-vector groups (P<0.05).Conclusion: SOX2 could have a certain promotive effect on the neural differentiation of DPSCs.

Key words: dental pulp stem cells, cell therapy, sex determining region Y-box 2, neural differentiation, neural progenitor cells, cell proliferation

中图分类号:

R329.2

袁静, 陈耀宇, 赵艺帆, 刘小博, 刘朋飞, 赵雷. SOX2对牙髓干细胞神经分化的促进作用及其意义[J]. 吉林大学学报(医学版), 2016, 42(06): 1076-1080.

YUAN Jing, CHEN Yaoyu, ZHAO Yifan, LIU Xiaobo, LIU Pengfei, ZHAO Lei. Promotive effect of SOX2 on neural differentiation of dental pulp stem cells and its significance[J]. Journal of Jilin University Medicine Edition, 2016, 42(06): 1076-1080.

参考文献

[1] Lewis S. Stem cells:Sending cells back in time[J]. Nat Rev Neurosci, 2013, 14(2):78.
[2] Trounson A, Thakar RG, Lomax G, et al. Clinical trials for stem cell therapiesv[J]. BMC Med, 2011, 9:52.
[3] Hendriks S, Dancet EAF, van Pelt AMM, et al. Artificial gametes:A systematic review of biological progress towards clinical application[J]. Hum Reprod Update, 2015, 21(3):285-296.
[4] Gronthos S, Mankani M, Brahim J, et al. Postnatal human dental pulp stem cells (dpscs) in vitro and in vivo[J]. PNAS, 2000, 97(25):13625-13630.
[5] Tatullo M, Marrelli M, Shakesheff KM, et al. Dental pulp stem cells:Function, isolation and applications in regenerative medicine[J]. Tissue Engineering Regenerat Med, 2015, 9(11):1205-1216.
[6] Paino F, La Noce M, Tirino V, et al. Histone deacetylase inhibition with valproic acid downregulates osteocalcin gene expression in human dental pulp stem cells and osteoblasts:Evidence for hdac2 involvement[J]. Stem Cells, 2014,32(1):279-289.
[7] Chang CC, Chang KC, Tsai SJ, et al. Neurogenic differentiation of dental pulp stem cells to neuron-like cells in dopaminergic and motor neuronal inductive media[J]. Formosan Med Assoc, 2014, 113(12):956-965.
[8] Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors[J]. Cell, 2006, 126(4):663-676.
[9] Takahashi K, Tanabe K, Ohnuki M, et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors[J]. Cell, 2007, 131(5):861-872.
[10] Cai J, Li W, Su H, et al. Generation of human induced pluripotent stem cells from umbilical cord matrix and amniotic membrane mesenchymal cells[J]. Biol Chem, 2010, 285(15):11227-11234.
[11] Esteban MA, Wang T, Qin B, et al. Vitamin C enhances the generation of mouse and human induced pluripotent stem cells[J]. Cell Stem Cell, 2010, 6(1):71-79.
[12] Tamaoki N, Takahashi K, Tanaka T, et al. Dental pulp cells for induced pluripotent stem cell banking[J]. J Dent Res, 2010, 89(8):773-778.
[13] Feng RP, Wen JH. Overview of the roles of sox2 in stem cell and development[J]. Biol Chem, 2015, 396(8):883-891.
[14] Thier M, Worsdorfer P, Lakes YB, et al. Direct conversion of fibroblasts into stably expandable neural stem cells[J]. Cell Stem Cell, 2012, 10(4):473-479.
[15] Liu P, Cai J, Dong D, et al. Effects of sox2 on proliferation, migration and adhesion of human dental pulp stem cells[J]. PLoS One, 2015, 10(10):e0141346.

SOX2对牙髓干细胞神经分化的促进作用及其意义

Promotive effect of SOX2 on neural differentiation of dental pulp stem cells and its significance

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