磁性Fe3O4纳米颗粒对大鼠脏器组织中Caveolin-1和Clathrin Heavy Chain蛋白表达的影响

doi:10.13481/j.1671-587x.20170210

吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (02): 260-265.doi: 10.13481/j.1671-587x.20170210

磁性Fe₃O₄纳米颗粒对大鼠脏器组织中Caveolin-1和Clathrin Heavy Chain蛋白表达的影响

孙维琦^1,2, 张晶², 靳曙光², 王洪艳², 李环², 孙志伟^1,3

1. 吉林大学公共卫生学院卫生毒理学教研室, 吉林长春 130021;
2. 北华大学公共卫生学院卫生毒理学教研室, 吉林吉林 132013;
3. 首都医科大学公共卫生学院卫生化学与毒理学系, 北京 100069

收稿日期:2016-10-10 出版日期:2017-03-28 发布日期:2017-03-31
通讯作者: 李环,副教授(Tel:0432-64608337,E-mail:jllihuan@126.com);孙志伟,教授,博士研究生导师(Tel:010-83911507,E-mail:zwsun@ccmu.edu.cn) E-mail:jllihuan@126.com;zwsun@ccmu.edu.cn
作者简介:孙维琦(1972-),女,吉林省吉林市人,讲师,医学博士,主要从事营养毒理学方面的研究。
基金资助:
吉林省教育厅科研基金资助课题[吉教科合字（2014）第211号；吉教科合字（2016）第71号]；吉林省卫生厅科研基金资助课题（2016J086）；北华大学青年教师提升计划基金资助课题（2016）

Influence of magnetic Fe₃O₄ nanoparticles in expressions of Caveolin-1 and Clathrin Heavy Chain proteins in organ tissues of rats

SUN Weiqi^1,2, ZHANG Jing², JIN Shuguang², WANG Hongyan², LI Huan², SUN Zhiwei^1,3

1. Department of Health Toxicology, School of Public Health, Jilin University, Changchun 130021, China;
2. Department of Health Toxicology, School of Public Health, Beihua University, Jilin 132013, China;
3. Department of Sanitary Chemistry and Toxicology, School of Public Health, Capital Medical University, Beijing 100069, China

Received:2016-10-10 Online:2017-03-28 Published:2017-03-31

摘要/Abstract

摘要： 目的：探讨磁性Fe₃O₄纳米颗粒对大鼠主要脏器组织中Caveolin-1及Clathrin Heavy Chain蛋白表达的影响，阐明其作用机制。方法：将24只Wistar大鼠按体质量随机分成对照组和低、中、高剂量磁性Fe₃O₄纳米颗粒组，尾静脉注射不同剂量磁性Fe₃O₄纳米颗粒24h后取脏器组织，Western blotting法检测大鼠主要脏器组织中Caveolin-1及Clathrin Heavy Chain蛋白的表达水平，荧光实时定量PCR法检测大鼠主要脏器组织中Caveolin-1及Clathrin Heavy Chain mRNA的表达水平。结果：与对照组比较，中和高剂量组大鼠肝脏和脾脏组织中Clathrin Heavy Chain蛋白和mRNA表达水平明显升高（P<0.05）。高剂量组大鼠肾脏组织中Clathrin Heavy Chain mRNA的表达水平与其他3组比较明显升高（P<0.05）。Caveolin-1蛋白表达水平在各剂量组之间比较差异无统计学意义（P>0.05）；与对照组比较，低、中和高剂量组大鼠肝脏、肺脏和脾脏组织中Caveolin-1 mRNA表达水平明显升高（P<0.05）；各组肾脏组织中Caveolin-1mRNA表达水平差异无统计学意义（P>0.05）。结论：磁性Fe₃O₄纳米颗粒能够诱导大鼠肝脏、肺脏、脾脏中Clathrin Heavy Chain蛋白表达增强，通过Clathrin Heavy Chain蛋白的内吞作用是磁性Fe₃O₄纳米颗粒进入大鼠肝脏、肺脏和脾脏细胞的途径之一。

关键词: Caveolin-1蛋白, Clathrin Heavy Chain蛋白, 磁性Fe₃O₄纳米颗粒

Abstract: Objective: To investigate the influence of magnetic Fe₃O₄ nanoparticles in the expressions of Caveolin-1 and Clathrin Heavy Chain proteins in organ tissues of the rats, and to clarify its mechanism. Methods: Twenty-four Wistar rats were randomly divided into control group, and low,medium and high doses of magnetic Fe₃O₄ nanoparticle groups by weights. 24 h after tail vein injection of different doses of magnetic Fe₃O₄ nanoparticles, the organ tissues were obtained. Western blotting method was used to detect the expressions of Caveolin-1 and Clathrin Heavy Chain proteins in the main organ tissues of the rats. Real-time fluorescent quantitative PCR was used to measure the expressions of Caveolin-1 and Clathrin Heavy Chain mRNA. Results: Compared with control group,the expressions levels Clathrin Heavy Chain protein and mRNA in liver and spleen tissues of the rats in medium and high doses groups were significantly increased (P<0.05).The expression level of Clathrin Heavy Chain mRNA in kidney tissue in high dose group was higher than those in other groups(P<0.05). The Caveolin-1 expression levels in the organ tissues of rats had no statistical differences between various groups (P>0.05).Compared with control group, the Caveolin-1 mRNA expression levels in liver, spleen, and lung tissues of the rats in low,medium and high doses groups were significantly increased (P<0.05);there were no statistical differences in the expression levels in kidney tissue of the rats between various groups(P>0.05). Conclusion: Magnetic Fe₃O₄ nanoparticles could enhance the expressions of Clathrin Heavy Chain in the liver, spleen, and lung tissues of the rats. Endocytosis of Clathrin Heavy Chain protein is one way for magnetic Fe₃O₄ nanoparticles into the liver, lung, spleen cells of the rats.

Key words: Clathrin Heavy Chain, magnetic Fe₃O₄ nanoparticles, Caveolin-1

中图分类号:

R994.6

孙维琦, 张晶, 靳曙光, 王洪艳, 李环, 孙志伟. 磁性Fe₃O₄纳米颗粒对大鼠脏器组织中Caveolin-1和Clathrin Heavy Chain蛋白表达的影响[J]. 吉林大学学报(医学版), 2017, 43(02): 260-265.

SUN Weiqi, ZHANG Jing, JIN Shuguang, WANG Hongyan, LI Huan, SUN Zhiwei. Influence of magnetic Fe₃O₄ nanoparticles in expressions of Caveolin-1 and Clathrin Heavy Chain proteins in organ tissues of rats[J]. Journal of Jilin University Medicine Edition, 2017, 43(02): 260-265.

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磁性Fe₃O₄纳米颗粒对大鼠脏器组织中Caveolin-1和Clathrin Heavy Chain蛋白表达的影响

Influence of magnetic Fe₃O₄ nanoparticles in expressions of Caveolin-1 and Clathrin Heavy Chain proteins in organ tissues of rats

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