粗毛纤孔菌对寒凝血瘀证大鼠血液流变学的影响及谱效关系分析

doi:10.13481/j.1671-587x.20180106

吉林大学学报(医学版) ›› 2018, Vol. 44 ›› Issue (01): 30-35.doi: 10.13481/j.1671-587x.20180106

粗毛纤孔菌对寒凝血瘀证大鼠血液流变学的影响及谱效关系分析

李庆杰¹, 包海鹰^1,2, 图力古尔¹, 李玉¹

1. 吉林农业大学食药用菌教育部工程研究中心, 吉林长春 130118;
2. 吉林农业大学中药材学院, 吉林长春 130118

收稿日期:2017-10-30 出版日期:2018-01-28 发布日期:2018-01-24
通讯作者: 包海鹰,教授,博士研究生导师(Tel:0431-84533358,E-mail:baohaiying2008@126.com) E-mail:baohaiying2008@126.com
作者简介:李庆杰(1981-),男,吉林省长春市人,在读农学博士,主要从事菌物方面的研究。
基金资助:
国家自然科学基金资助课题（31270088）；教育部长江学者和创新团队发展计划项目资助课题（IRT-15R25）

Influence of Inonotus hispidus in hemorheology in rat model with blood stasis due to cold syndrome and analysis on spectral efficiency

LI Qingjie¹, BAO Haiying^1,2, BAU Tolgor¹, LI Yu¹

1. Engineering Research Center for Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, China;
2. College of Chinese Medicinal Meterial, Jilin Agricultural University, Changchun 130118, China

Received:2017-10-30 Online:2018-01-28 Published:2018-01-24

摘要/Abstract

摘要： 目的：观察粗毛纤孔菌子实体对寒凝血瘀证大鼠血液流变学的影响，探讨粗毛纤孔菌的活血化瘀作用。方法：Wistar大鼠随机分为正常对照组、血瘀模型组、阳性对照组和低、高剂量粗毛纤孔菌子实体（S1~S6）组，共15组，每组12只。各组大鼠连续灌给药7d（正常对照组和模型组大鼠给予蒸馏水），除正常对照组外，其余各组大鼠均注射盐酸肾上腺素和浸入冰水中造成寒凝血瘀证模型。检测大鼠血液流变学指标，并进行谱效分析。结果：寄生于不同树种的粗毛纤孔菌的黄色子实体（S3~S6）均具有改善寒凝血瘀证模型大鼠血液流变学作用，与模型组比较，高剂量粗毛纤孔菌黄色子实体组大鼠血浆黏度和全血黏度均降低（P<0.05）。粗毛纤孔菌的黑色子实体（S1和S2）具有与黄色子实体相似的功效，但与模型组比较，粗毛纤孔菌黑色子实体组大鼠血液流变学指标无明显变化（P>0.05）。谱效分析，11个色谱峰对应的成分与大鼠血浆黏度变化密切相关，相关系数均>0.8；经鉴定，其中4个化合物为phellibaumin A、phelligridin C（反式）、phelligridin D和3'4'-dihydroxy-5-[（11-hydroxyphenyl）-6，7-vinyl]-3，5-dioxa-fluoren-5-one。结论：寄生于不同树种的粗毛纤孔菌的黄色子实体均可以降低大鼠血浆黏度和全血黏度，具有活血化瘀作用。

关键词: 粗毛纤孔菌, 血液流变学, 谱效分析, 寒凝血瘀证

Abstract: Objective: To explore the effects of the activating circulation to remove blood stasis through observingthe influence of Inonotus hispidus on hemorheology in the rat models with blood stasis due to cold syndrome.. Methods: The rats were randomly divided into normal control group, blood stasis model group, positive control group, low and high doses ofInonotus hispidus(S1-S6) sample groups; there were 15 groups,and 12 rats were in each group. Except for normal control group, the rats in other groups were injected adrenaline hydrochloride and immersing in ice water to build the blood stasis due to cold syndrome rat models.The indexes of hemorheoloy of the rats were detected,and the HPLC spectrum-effect relationship was analyzed. Results: The yellow fruiting bodies(S3-S6) of Inonotus hispidus which were parasitic on the different trees significantly improved the hemorheological indexes in the rat models.Compared with model group, the blood viscosity and whole blood viscosity of the rats in high dose of yellow fruiting body of Inonotus hispidus group were decreased(P<0.05).The black fruiting body of Inonotus hispidus showed the similar effect to the yellow fruiting body, but the hemorheological indexes of the rats in black fruiting body of Inonotus hispidus group had no significant changes compared with model group(P>0.05). The results of the spectrum-effect showed that the corresponding components of 11 chromatographic peaks were closely correlated with the changes of plasma viscosity, and the correlation coefficient > 0.8.Through identification,four compounds of them were named phellibaumin A,phelligridin C(cis),phelligridin C(trans),phelligridin D and 3'4'-dihydroxy-5-[(11-hydroxyphenyl)-6,7-vinyl]-3,5-dioxa-fluoren-5-one. Conclusion: The yellow fruiting bodies of Inonotus hispidus which are parasitic on the different trees can decrease the blood viscosity and whole blood viscosity and have the effects of activating the circulation to remove blood stasis.

Key words: spectrum-effect relationship, blood stasis due to cold syndrome, hemorheology, Inonotus hispidus

中图分类号:

R285.5

李庆杰, 包海鹰, 图力古尔, 李玉. 粗毛纤孔菌对寒凝血瘀证大鼠血液流变学的影响及谱效关系分析[J]. 吉林大学学报(医学版), 2018, 44(01): 30-35.

LI Qingjie, BAO Haiying, BAU Tolgor, LI Yu. Influence of Inonotus hispidus in hemorheology in rat model with blood stasis due to cold syndrome and analysis on spectral efficiency[J]. Journal of Jilin University Medicine Edition, 2018, 44(01): 30-35.

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粗毛纤孔菌对寒凝血瘀证大鼠血液流变学的影响及谱效关系分析

Influence of Inonotus hispidus in hemorheology in rat model with blood stasis due to cold syndrome and analysis on spectral efficiency

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