“血瘀证”下急性心肌梗死模型与单纯急性心肌梗死模型大鼠血液生化学比较

J4 ›› 2010, Vol. 36 ›› Issue (3): 510-513.

“血瘀证”下急性心肌梗死模型与单纯急性心肌梗死模型大鼠血液生化学比较

曲绍春|于晓风|王佳|周金影|谢昊霖|睢大筼

吉林大学药学院药理学教研室|吉林 |长春130021

收稿日期:2009-08-27 出版日期:2010-05-28 发布日期:2010-05-28
通讯作者: 睢大筼（Tel：0431-85619705，E-mail: suidy＠jlu.edu.cn） E-mail:suidy＠jlu.edu.cn
作者简介:曲绍春（1954-）|男|吉林省长春市人|副主任技师|主要从事心血管药理学实验研究。
基金资助:
吉林省科技厅新药基金项目资助课题（20050916-1）

Comparison of |blood biochemics between |acute myocardial infarction models with blood stasis and |simple acute myocardial infarction models in rats

QU Shao-Chun, YU Xiao-Feng, WANG Jia, ZHOU Jin-Ying, XIE Hao-Lin, SUI Da-Yuan

Department of Pharmacology,School of Pharmacy,Jilin University,Changchun130021,China

Received:2009-08-27 Online:2010-05-28 Published:2010-05-28

摘要/Abstract

摘要：

目的：建立大鼠 “血瘀证”下急性心肌梗死模型，探讨其与单纯急性心肌梗死模型在血液生化学方面的差异，为评价治疗胸痹心痛中药的药效学提供理论依据。方法：Wistar大鼠随机分为空白对照组、急性血瘀模型组、急性心肌梗死假手术组、单纯急性心肌梗死模型组及“血瘀证”下急性心肌梗死模型组（每组12只），在大鼠急性 “血瘀”情况下复制急性心肌梗死模型，测定各组大鼠血清丙二醛（MDA）、一氧化氮（NO）、游离脂肪酸（FFA）及肿瘤坏死因子α（TNF-α）含量，血清超氧化物歧化酶（SOD）及谷胱甘肽过氧化物酶（GSH-Px）活性，血浆前列环素（PGI2）、血栓素A2（TXA2）及内皮素（ET）水平，并与单纯急性心肌梗死模型大鼠进行比较。结果： “血瘀证”下急性心肌梗死模型大鼠血清MDA、SOD、GSH-Px及FFA水平与单纯急性心肌梗死模型大鼠比较差异无显著性（P>0.05），PGI2及NO的减少程度和TXA2、ET及TNF-α的增加程度均明显大于单纯急性心肌梗死模型组（P<0.05），TNF-α活性明显高于单纯急性心肌梗死模型组（P<0.05）。结论：大鼠“血瘀证”下急性心肌梗死模型与单纯急性心肌梗死模型在自由基对心肌的损伤及心肌缺血时FFA 的代谢紊乱方面无明显区别，但在内源性血管活性物质的释放方面存在明显区别。提示在评价治疗胸痹心痛中药的药效学时，仅采用单纯的急性心肌梗死模型，不考虑中医的证候是有缺陷的。

关键词: 急性血瘀；急性心肌梗死；疾病模型, 动物；自由基；血管活性物质

Abstract:

Abstract:Objective To construct the acute myocardial infarction models in rats with blood stasis and study the difference on blood biochemics between the acute myocardial infarction models with blood stasis and the simple acute myocardial infarction models.Methods Wistar rats were randomly divded into control group, acute blood stasis model group,acute myocardial infarction sham operation group, acute myocardial infarction model group and of acute myocardial infarction model with blood stasis group.The acute myocardial infarction models under the status of the acute blood stasis in rats were set up.The serum malondialdehyde (MDA),nitric oxide(NO),free fatty acid (FFA),tumor necrosis factor-α (TNF-α) levels were detected,the activities of serum superoxide dismutase (SOD),glutathione peroxidase (GSH-Px) and the levels of prostacycline(PGI2),thromboxane A2 (TXA2) and endothelin (ET) in plasma were determined.Results There were not obvious differences in MDA,SOD,GSH-Px and FFA between the acute myocardial infarction models with blood stasis in rats and the simple acute myocardial infarction models(P<0.05).The decrease levels of PGI2 and NO,and the increase extents of TXA2,ET and TNF-α in the acute myocardial infarction models in rats with blood stasis were higher than those in the simple acute myocardial infarction models(P<0.05).Conclusion The changes of the myocardial damage induced by oxygen free radical and the metabolic disorder of FFA resulted from the myocardial ischemia are not significant,while the releasing contents of interior active mature,such as ET,FFA,TXA2 and NO,are significant when the acute myocardial infarction models in rats with blood stasis and the simple acute myocardial infarction models are compared.The results show that it is defective to evaluate pharmacodynamics of traditional Chinese drug with only simple acute myocardial infarction models.

Key words: acute blood stasis, acute myocardial infarction, disease model,animal;free , radicals;vasoactive factors

中图分类号:

R542.2

曲绍春, 于晓风, 王佳, 周金影, 谢昊霖, 睢大筼. “血瘀证”下急性心肌梗死模型与单纯急性心肌梗死模型大鼠血液生化学比较[J]. J4, 2010, 36(3): 510-513.

QU Shao-Chun, YU Xiao-Feng, WANG Jia, ZHOU Jin-Ying, XIE Hao-Lin, SUI Da-Yuan. Comparison of |blood biochemics between |acute myocardial infarction models with blood stasis and |simple acute myocardial infarction models in rats[J]. J4, 2010, 36(3): 510-513.