静电喷雾法制备聚乳酸-羟基乙酸共聚物/羟基磷灰石微载体的工艺及其效果评价

doi:10.13481/j.1671-587x.20190241

吉林大学学报(医学版) ›› 2019, Vol. 45 ›› Issue (02): 439-444.doi: 10.13481/j.1671-587x.20190241

• 方法学 • 上一篇

静电喷雾法制备聚乳酸-羟基乙酸共聚物/羟基磷灰石微载体的工艺及其效果评价

赵一安¹, 杨舒婷², 李亚龙³, 刘国民³, 孙淑芬⁴, 罗云纲¹

1. 吉林大学第二医院口腔科, 吉林长春 130041;
2. 吉林大学口腔医院修复科, 吉林长春 130021;
3. 吉林大学第二医院骨科, 吉林长春 130041;
4. 吉林大学口腔医院牙体牙髓科, 吉林长春 130021

收稿日期:2018-06-25 发布日期:2019-03-29
通讯作者: 罗云纲,副教授,博士研究生导师(Tel:0431-81136882,E-mail:luoyungang@sohu.com) E-mail:luoyungang@sohu.com
作者简介:赵一安(1992-),女,吉林省吉林市人,在读医学硕士,主要从事口腔种植修复方面的研究。
基金资助:
吉林省财政厅省直卫生专项项目资助课题（3D516D703429）

Preparation process of polylactic acid-glycolic acid copolymer/hydroxyapatite microcarrier by electrostatic spraying method and its effect evaluation

ZHAO Yian¹, YANG Shuting², LI Yalong³, LIU Guomin³, SUN Shufen⁴, LUO Yungang¹

1. Department of Stomatology, Second Hospital, Jilin University, Changchun 130041, China;
2. Department of Prosthodontics, Stomatology Hospital, Jilin University, Changchun 130021, China;
3. Department of Orthopedics, Second Hospital, Jilin University, Changchun 130041, China;
4. Department of Endodontics and Operative Dentistry, Stomatology Hospital, Jilin University, Changchun 130021, China

Received:2018-06-25 Published:2019-03-29

摘要/Abstract

摘要： 目的：探讨静电喷雾法制备聚乳酸-羟基乙酸共聚物/羟基磷灰石（PLGA/HA）微载体的工艺过程，阐明PLGA/HA微载体的优势表征。方法：采用HA （20 nm，99.9%）和PLGA （LA/GA=50/50，Mw30k），利用静电喷雾法制备PLGA/HA微载体，观察不同HA质量浓度（1%、3%、5%）、不同电压（4.0、4.5、>5.0kV）对微载体形貌的影响，获得最佳制球参数。以PLGA微载体为PLGA组，PLGA+1% HA为1% PLGA/HA组，PLGA+3% HA为3% PLGA/HA组，PLGA+5% HA为5% PLGA/HA组，单纯细胞作为空白对照组。采用扫描电镜（SEM）、细胞增殖实验、细胞荧光染色实验和傅里叶红外光谱（FTIR）等检测PLGA/HA微载体表征。结果：SEM观察，PLGA/HA微载体颗粒均匀，均为椭圆形或圆形，无异常形态球，表面光滑，无锐利边缘，球体之间无粘连、无聚集，不同浓度配比微载体之间无明显差别。细胞增殖实验检测，黏附细胞数量5% PLGA/HA组 > 3% PLGA/HA组 > 1% PLGA/HA组 > PLGA组 > 空白对照组（P<0.05），细胞数量随HA浓度增加而升高，5% PLGA/HA组微载体对细胞亲和性最佳，微载体无细胞毒性。细胞荧光染色实验检测，MC3T3-E1细胞在微载体上黏附状态良好。FTIR检测显示HA特征吸收峰，表明复合微载体中含有PLGA与HA。结论：成功利用静电喷雾技术建立PLGA/HA微载体的制备工艺，该方法操作简单便捷，制球效果优良，在骨组织工程方面有广泛应用前景。

关键词: 静电喷雾, 微载体, 聚乳酸-羟基乙酸共聚物, 羟基磷灰石, 骨组织工程

Abstract: Objective: To investigate the preparation process of polylactic acid-glycolic acid copolymer/hydroxyapatite (PLGA/HA) microcarriers by electrostatic spraying method, and to elucidate the superiority of PLGA/HA microcarriers.Methods: The PLGA/HA microcarriers were prepared by electrostatic spraying method using nano-HA (20 nm, 99.9%) and PLGA (LA/GA=50/50, Mw30k),and the influence of different concentrations (1%, 3%, 5%) and different voltages (4.0, 4.5, > 5.0 kV) of HA in the morphology of the microcarriers was investigated and the best ball making parameters were obtained.The PLGA microcarriers were used as PLGA group, PLGA+1%HA as 1% PLGA/HA group, PLGA+3% HA as 3% PLGA/HA group, PLGA+5%HA as 5%PLGA/HA group, and the simple cells were used as blank control group.The characteristics of PLGA/HA microcarriers were detected by scanning electron microscope (SEM), cell proliferation test, cell fluorescence staining experiment, and Fourier transform infrared spectroscopy (FTIR).Results: The SEM results showed that the microcarrier particles were uniform, all of them were elliptical or circular, without abnormal shape spheres, with smooth surface, without sharp edges, adhesion between the spheres and aggregation, and there were no significant differences between the different concentrations of microcarriers.The cell proliferation test results showed that the order of adhesion cells was 5%PLGA/HA group > 3%PLGA/HA group > 1% PLGA/HA group > PLGA group > blank control group (P<0.05); the number of cells was increased with the increasing of HA concentration; the microcarriers in 5%PLGA/HA group had the best cell affinity and the microcarriers had no cytotoxity.The cell fluorescence staining experiment showed that the MC3T3-E1 cells adhered well on the microcarriers.The FTIR analysis results showed that HA characteristic absorption peak was observed, indicating that the composite microcarrier contained PLGA and HA.Conclusion: The preparation process of PLGA/HA microcarriers is successfully established by electrostatic spraying method.The method is simple and convenient to operate, and has excellent ball-making effect.It has broadly application prospects in bone tissue engineering.

Key words: electrostatic spraying, microcarriers, polylactic acid-glycolic acid copolymer, hydroxyapatite, bone tissue engineering

中图分类号:

R331

赵一安, 杨舒婷, 李亚龙, 刘国民, 孙淑芬, 罗云纲. 静电喷雾法制备聚乳酸-羟基乙酸共聚物/羟基磷灰石微载体的工艺及其效果评价[J]. 吉林大学学报(医学版), 2019, 45(02): 439-444.

ZHAO Yian, YANG Shuting, LI Yalong, LIU Guomin, SUN Shufen, LUO Yungang. Preparation process of polylactic acid-glycolic acid copolymer/hydroxyapatite microcarrier by electrostatic spraying method and its effect evaluation[J]. Journal of Jilin University(Medicine Edition), 2019, 45(02): 439-444.

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静电喷雾法制备聚乳酸-羟基乙酸共聚物/羟基磷灰石微载体的工艺及其效果评价

Preparation process of polylactic acid-glycolic acid copolymer/hydroxyapatite microcarrier by electrostatic spraying method and its effect evaluation

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