变形链球菌耐氟菌株eriCF基因差异性表达及其意义

doi:10.13481/j.1671-587x.20190314

吉林大学学报(医学版) ›› 2019, Vol. 45 ›› Issue (03): 551-557.doi: 10.13481/j.1671-587x.20190314

• 基础研究 • 上一篇

变形链球菌耐氟菌株eriC^F基因差异性表达及其意义

曲添, 张红, 张志民, 杜奥博, 邵思奇, 杨瑶瑶, 刘璐

吉林大学口腔医院牙体牙髓科, 吉林长春 130021

收稿日期:2018-11-28 发布日期:2019-06-05
通讯作者: 张志民,教授,博士研究生导师(Tel:0431-85579500,E-mail:zhangzm1964@sina.com);张红,主治医师(Tel:0431-88796017,E-mail:zhanghong1983@jlu.edu.cn) E-mail:zhangzm1964@sina.com;zhanghong1983@jlu.edu.cn
作者简介:曲添(1993-),女,吉林省通化市人,在读医学硕士,主要从事变形链球菌致龋作用方面的研究。
基金资助:
吉林省科技厅自然科学基金资助课题（20160101003JC）；吉林省教育厅"十三五"科学技术项目资助课题（3D518Q183431）

Differential expression of eriC^F gene in fluoride-resistant strains of Streptococcus mutans and its significance

QU Tian, ZHANG Hong, ZHANG Zhimin, DU Aobo, SHAO Siqi, YANG Yaoyao, LIU Lu

Department of Operative and Endodontics, Stomatology Hospital, Jilin University, Changchun 130021, China

Received:2018-11-28 Published:2019-06-05

摘要/Abstract

摘要： 目的：探讨不同氟浓度条件下eriC^F1和eriC^F2基因对变形链球菌（S.mutans）耐氟菌株UA159-FR氟抗性的影响，阐明eriC^F基因差异性表达与S.mutans耐氟菌株抗性的关系。方法：重组大肠杆菌感受态细胞BL21分为eriC^F1组（eriC^F1+Peasy-Blunt E2）、eriC^F2组（eriC^F2+Peasy-Blunt E2）和KZ组（Peasy-Blunt E2）。基因克隆和IPTG诱导后，检测3组EriC^F1和EriC^F2蛋白表达水平，并分别测定3组菌株在不同氟环境（1.5、2.0、2.5和3.0 g·L^-1氟化钠）下的菌液浓度和菌株生长速度，绘制菌株的生长曲线。结果：在对数期（8 h）和稳定期（16 h），3组菌株在不同氟环境下（1.5、2.0、2.5和3.0 g·L^-1氟化钠）的菌液终浓度及菌株生长速度组内比较，3组菌株菌液终浓度和生长速度均随着氟化钠浓度升高而降低；组间比较，相同氟化钠浓度条件下，菌液终浓度和生长速度均为eriC^F2组 > eriC^F1组 > KZ组（P<0.05或P<0.01）；与eriC^F1组比较，eriC^F2组菌液浓度均明显升高（P<0.01）。结论：eriC^F1和eriC^F2基因均能增强大肠杆菌的氟抗性，且表达eriC^F2基因比表达eriC^F1基因的大肠杆菌的氟抗性更强。

关键词: eriC^F1, eriC^F2, 变形链球菌, 变形链球菌耐氟菌株, 氟抗性

Abstract: Objective:To explore the effects of eriC^F1 and eriC^F2 genes under the condition of different fluoride concentrations on the fluoride resistance of Streptococcus mutans(S.mutans) fluoride-resistant strain UA159-FR, and to elucidate the relationship between the differential expressions of eriC^F genes and the fluoride resistance of S.mutans fluoride-resistant strain. Methods:The recombinant E.coli competent cells BL21 were divided into eriC^F1 group (eriC^F1 + Peasy-Blunt E2), eriC^F2 group (eriC^F2 + Peasy-Blunt E2) and KZ group (Peasy-Blunt E2). After gene cloning and IPTG induction, the expression levels of EriC^F1 and EriC^F2 proteins in three groups were detected, and the concentrations of bacterial solution and the growth rates of bacterium of strains in three groups were determined under different concentrations of fluoride conditions (1.5,2.0,2.5,and 3.0 g·L^-1 sodium fluoride);the growth curves were drawn. Results:At logarithmic phase(8 h) and stable phase(16 h), the final concentrations of bacterial solution and the growth rates of the strains in three groups under different concentrations of fluorine environments (1.5,2.0,2.5 and 3.0 g·L^-1 sodium fluoride) were compared in the same group,and the final concentrations and the growth rates of bacterial solution of the strains in three groups were decreased with the increasing of sodium fluoride concentration; the final concentrations and the growth rates of bacterial solution results were eriC^F2 group > eriC^F1 group > KZ group (P<0.05 or P<0.01).compared between three groups under the same concentration of sodium fluoride, the concentration of bacterial solution in eriC^F2 group was increased significantly compared with eriC^F1 group(P<0.01). Conclusion:Both eriC^F1 and eriC^F2 genes can enhance the fluoride resistance of E.coli, and the fluoride resistance of E.coli expressing eriC^F2 gene is stronger than that of E.coli expressing eriC^F1 gene.

Key words: eriC^F1, eriC^F2, Streptococcus mutans, fluoride-resistant strains of Streptococcus mutans, fluorine resistance

中图分类号:

378.12

曲添, 张红, 张志民, 杜奥博, 邵思奇, 杨瑶瑶, 刘璐. 变形链球菌耐氟菌株eriC^F基因差异性表达及其意义[J]. 吉林大学学报(医学版), 2019, 45(03): 551-557.

QU Tian, ZHANG Hong, ZHANG Zhimin, DU Aobo, SHAO Siqi, YANG Yaoyao, LIU Lu. Differential expression of eriC^F gene in fluoride-resistant strains of Streptococcus mutans and its significance[J]. Journal of Jilin University(Medicine Edition), 2019, 45(03): 551-557.

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变形链球菌耐氟菌株eriC^F基因差异性表达及其意义

Differential expression of eriC^F gene in fluoride-resistant strains of Streptococcus mutans and its significance

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变形链球菌耐氟菌株eriCF基因差异性表达及其意义

Differential expression of eriCF gene in fluoride-resistant strains of Streptococcus mutans and its significance

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变形链球菌耐氟菌株eriC^F基因差异性表达及其意义

Differential expression of eriC^F gene in fluoride-resistant strains of Streptococcus mutans and its significance