中国北方汉族大学生TLR4基因多态性与肺功能和体重指数的关联性分析

J4 ›› 2010, Vol. 36 ›› Issue (4): 687-690.

中国北方汉族大学生TLR4基因多态性与肺功能和体重指数的关联性分析

朱铁峰¹, 刘馨梅²|姚英春³, 李一帆⁴

1. 长春中医药大学体育教学部|吉林长春 130117；2. 广东省深圳市凯杰生物工程有限公司|广东深圳 518057；3. 吉林华桥外国语学院|吉林长春 130117；4. 长春中医药大学解剖学教研室|吉林长春 130117

收稿日期:2010-03-02 出版日期:2010-07-28 发布日期:2010-07-28
通讯作者: 朱铁峰（Tel: 0431-85911686，E-mail：zww_zy@sina.com） E-mail:zww_zy@sina.com
作者简介:朱铁峰（1977-）|男|吉林省长春市人|讲师|教育硕士|主要从事运动医学研究。
基金资助:
国家自然科学基金资助课题（30870584）

Relevance analysis on TLR4 polymorphism with lung function and body mass index in Han population from North China

ZHU Tie-Feng¹, LIU Xin-Mei², YAO YIng-Chun³, LI Yi-Fan⁴

1.Department of Physical Education,Changchun University of Chinese Medicine, Changchun 130117,China;2. QIAGEN Co.,Ltd,Shenzhen 518057,China;3. Jilin Huaqiao Foreign Languages Institute,Changchun 130117,China;4.Department of Anatomy,Changchun University of Chinese Medicine,Changchun 130117,China

Received:2010-03-02 Online:2010-07-28 Published:2010-07-28

摘要/Abstract

摘要：

目的：探讨中国北方汉族大学生9q33 区域TLR4 基因多态性与肥胖及肺功能的关系。方法：采用漱口水提取基因组DNA方法，收集506 例中国北方汉族大学生样品，采用聚合酶链式反应-限制性片段长度多态性（PCR-RFLP）方法，检测TLR4 基因rs2149356 单核苷酸多态性（SNP）位点基因型。应用拟合优度χ2检验分析基因型分布频率是否符合Hardy-Weinberg 平衡定律，应用t 检验、χ2 检验和数量性状分析分别进行肺功能比较及等位基因和基因型关联性分析。结果：独立样本t检验分析表明，男、女肥胖组和对照组肺活量差异无显著性（P>0.05）。肥胖组和对照组rs2149356 位点基因型分布频数均符合Hardy、-Weinberg 平衡（P>0.05）。等位基因关联分析表明，肥胖组和对照组等位基因A 和C 的频数分布差异无显著性（P>0.05）；基因型关联分析表明，病例组和对照组A/A、C/A 和C/C 3 种基因型的频数分布差异无显著性（P>0.05）；不同肺活量组rs2149356 位点基因型分布频数均符合Hardy-Weinberg 平衡（P>0.05）。结论：肥胖组和对照组肺活量无显著差异，TLR4 基因rs2149356 位点可能与中国北方汉族肥胖及肺活量无关，但是不能排除该基因其他SNPs 位点与肥胖及肺活量有关联。

关键词: 肥胖；肺功能；单核苷酸多态性；TLR4；rs2149356

Abstract:

Objective
To investigate the genetic association between TLR4 gene in chromosome 9q33 and obesity and lung function in Han population from North China.Methods Genomic DNA was isolated from the saliva samples.A single nucleotide polymorphism,rs2149356 (TspEⅠsite) present in the TLR4 gene,was detected using PCR-based restriction fragment length polymorphism(PCR-RFLP) analysis among 506 Chinese Han population.The Hardy-Weinberg equilibrium for genotypic distribution was estimated by the goodness-of-fit χ2 test.The analysis of forced vital capacity (FVC) and the frequencies of alleles and genotypes of TLR4 gene and the relationship between allelic frequencies and obesity and lung function were statistically computed.Results The result of t test did not show significant difference of FVC between obesity group and control(P>0.05).The result of goodness-of-fit χ2 test showed that the genotypic distributions of rs2149356 were not deviated from Hardy-Weinberg equilibrium in various groups (P>0.05).The relevance analysis on allelic gene showed that there were no significant differences in the distributions of the frequencies of allelic A and allelic C between control and obesity groups (P>0.05).The result of χ2 test did not show allelic association and genotypic association for rs2149356 between obesity and FVC (P>0.05).The result of goodness-of-fit χ2 test showed that the genotypic distributions of rs2149356 were not deviated from Hardy-Weinberg equilibrium in FVC variant groups(P>0.05),too.Conclusion rs2149356 locus may not be associated with obesity and lung function,but the correlation between the other SNPs locus at TLR4 gene and obesity and lung function could not be ruled out.

Key words: obesity;lung function;single nucleotide polymorphism;rs2149356

中图分类号:

朱铁峰, 刘馨梅, 姚英春, 李一帆. 中国北方汉族大学生TLR4基因多态性与肺功能和体重指数的关联性分析[J]. J4, 2010, 36(4): 687-690.

ZHU Tie-Feng, LIU Xin-Mei, YAO YIng-Chun, LI Yi-Fan. Relevance analysis on TLR4 polymorphism with lung function and body mass index in Han population from North China[J]. J4, 2010, 36(4): 687-690.