MIF和VEGF在卵巢癌组织中的表达及其与微血管密度的关系

J4 ›› 2010, Vol. 36 ›› Issue (4): 713-716.

MIF和VEGF在卵巢癌组织中的表达及其与微血管密度的关系

赵强¹, 周彤^*, 翟颖仙², 王建伟², 靳朝晖³, 石英爱²

1.吉林大学第一医院儿外科|吉林长春 130021；2.吉林大学基础医学院病理生物学教育部重点实验室|吉林长春 130021；3.吉林省长春市中心医院心血管内科|吉林长春 130051

收稿日期:2010-04-21 出版日期:2010-07-28 发布日期:2010-07-28
通讯作者: 石英爱（Tel:0431-85619481，E-mail:shiya@jlu.edu.cn） E-mail:shiya@jlu.edu.cn
作者简介:赵强（1977-）|男|吉林省长春市人|医学硕士|主要从事肿瘤生物学研究。 
基金资助:
吉林省科技厅科技发展计划项目资助课题（200705369，200705373）

Expressions of MIF and VEGF in ovarian cancer tissues and their correlations with microvascular density


ZHAO Qiang¹, ZHOU Tong^*, ZHAI Ying-Xian², WANG Jian-Wei², JIN Zhao-Hui³, SHI Ying-Ai²

1.Department of Pediatric Surgery,First Hospital,Jilin University,Changchun 130021,China;2. Key Laboratory of Pathobiology,Ministry of Education,School of Basic Medical Sciences,Jilin University,Changchun 130021,China;3.Department of Cardiology,Changchun Central Hospital,Changchun 130051,China

Received:2010-04-21 Online:2010-07-28 Published:2010-07-28

摘要/Abstract

摘要：

目的：观察巨噬细胞移动抑制因子（MIF）和血管内皮细胞生长因子（VEGF）在卵巢癌组织中的表达并计数微血管密度（MVD），探讨MIF和VEGF与卵巢癌发生的关系及在卵巢癌血管生成中的作用。方法：利用免疫组织化学染色技术检测MIF、VEGF和第八因子相关抗原在卵巢癌组织和正常卵巢组织中的表达；采用Image Pro Plus（IPP）6.0图像分析软件对结果进行分析。结果：MIF和VEGF在正常卵巢组织的实质细胞中不表达，在卵巢癌肿瘤细胞的胞浆呈阳性表达，且随着卵巢癌分化程度的降低，表达强度逐渐增强；低分化卵巢癌与高分化和中分化卵巢癌比较差异有显著性（P<0.05或P<0.01）。低分化卵巢癌组织中的MVD(50.83±11.59)明显增加，与正常卵巢组织(18.16±2.26)、高分化(19.88±3.92)及中分化(31.1±3.08)卵巢癌组织比较差异有显著性（均P<0.01）。MIF表达与VEGF表达呈正相关（r=0.579　7，P<0.01），MIF表达与卵巢癌MVD呈正相关（r=0.962　3，P<0.001）。结论：MIF的表达与卵巢癌的分化程度密切相关，MIF可能通过上调VEGF表达促进卵巢癌的肿瘤血管生成。

关键词: 巨噬细胞移动抑制因子；血管内皮细胞生长因子；微血管密度；血管新生；卵巢肿瘤

Abstract:

Objective
To investigate the expressions of macrophage migration inhibitory factor (MIF) and vascular endothelial growth factor (VEGF) in ovarian cancer tissues and detect microvascular density (MVD) and elucidate the correlations between MIF,VEGF and the occurrence of ovarian cancer and explore their roles in tumor angiogenesis.Methods The expressions of MIF,VEGF and factor Ⅷ related antigen in normal ovarian tissues and ovarian cancer tissues were detected by immunohistochemical staining.The results were analyzed by Image Pro Plus（IPP）6.0 software.Results There were no visible expressions of MIF and VEGF in normal ovarian tissues,but positive expressions in ovarian cancer.The expressions of MIF and VEGF were increased significantly with the decreasing grade of the ovarian cancer.There were significant differences of the IOD values between well-differentiated,moderately differentiated and poorly differentiated ovarian cancer（P<0.05 or P<0.01）.The MVD in poorly differentiated ovarian cancer tissues (50.83±11.59) was significantly higher than those in normal ovarian tissues(18.16±2.26),well differentiated(19.88±3.92),moderately differentiated(31.1±3.08) ovarian cancer tissues(all P<0.01).There was positive correlation between the expressions of MIF and VEGF in ovarian cancer tissues (r=0.579　7，P<0.01).Moreover,the expression of MIF was positively correlated with MVD in ovarian cancer tissues (r=0.962　3，P<0.01).Conclusion MIF plays an important role in the oncogenesis and development of ovarian cancer,and it may promote the angiogenesis of ovarian cancer tissues by up-regulating the expression of VEGF.

Key words: macrophage migration inhibitory factor;vascular endothelial growth factor;microvascular density;angiogenesis;ovarian cancer

中图分类号:

R737.31

赵强, 周彤, 翟颖仙, 王建伟, 靳朝晖, 石英爱. MIF和VEGF在卵巢癌组织中的表达及其与微血管密度的关系[J]. J4, 2010, 36(4): 713-716.

ZHAO Qiang, ZHOU Tong, ZHAI Ying-Xian, WANG Jian-Wei, JIN Zhao-Hui, SHI Ying-Ai. Expressions of MIF and VEGF in ovarian cancer tissues and their correlations with microvascular density
[J]. J4, 2010, 36(4): 713-716.

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