脐带血中血管内皮前期细胞的分离

doi:教育部博士专项基金

脐带血中血管内皮前期细胞的分离

马丽萍¹，李伟¹，李玉林^1*，张丽红¹，邵明玉²

1. 吉林大学基础医学院病理解剖学教研室，吉林长春，130021；2. 吉林大学基础医学院免疫学教研室，吉林长春，130021

收稿日期:2003-01-05 修回日期:1900-01-01 出版日期:2004-01-28 发布日期:2004-01-28

Isolation of endothelial progenitor cells fromumbilical cord blood

MA Li-ping¹, LI Wei¹，LI Yu-lin^1*, ZHANG Li-hong¹, SHAO Ming-yu²

1.　Department of Pathology, School of Basic Medical Sciences,Jilin University, Changchun 130021，China;2. Department of Immunology， School of Basic Medical Sciences,Jilin University, Changchun 130021，China

Received:2003-01-05 Revised:1900-01-01 Online:2004-01-28 Published:2004-01-28

摘要/Abstract

摘要： 目的：从脐带血中分离单个核细胞以获得血管内皮前期细胞，并观察其生物学行为。方法：用密度为1.077的淋巴细胞分离液分离脐带血中单个核细胞,种植在铺有纤维连接素的培养板中，3 d后去除未附着细胞，观察附着细胞的生长情况，采用免疫组化法进行鉴定。结果：单个核细胞培养3 d后可见大量的附着细胞和少量梭形细胞，梭形细胞数量在第7天时达到高峰。附着细胞对第八因子相关抗原呈阳性反应。结论：在纤维连接素上培养来源于脐带血的单个核细胞可获得血管内皮前期细胞。

关键词: 细胞学, 血管内皮前期细胞, 细胞培养, 纤连蛋白类, von Willebrand因子

Abstract: Objective To isolate endothelial progenitor cells from umbilical cord blood and observe their biologic behaviors. Methods The mononuclear cells were isolated from umbilical cord blood using Ficoll gradients, and then were cultured on fibronectin. The characteristics of the attached cells were got and they were subjected to immunocytochemical method to analyze the expression of von Willebrand factor (vWF). Results After the mononuclear cells were cultured for 3 days, a large amount of attached cells and some spindle-shaped cells were observed. The number of spindle-shaped cells increased until 7th day. The immunocytochemical method showed that the attached cells were positively stained for vWF. Conclusion The endothelial progenitor cells may be isolated from umbilical cord blood.

Key words: cytology, endothelial progenitor cells, cell culture, fibronectins, von Willebrand factor

中图分类号:

Q813.1

马丽萍，李伟，李玉林，张丽红，邵明玉. 脐带血中血管内皮前期细胞的分离[J]. J4, 2004, 30(1): 150-152.

MA Li-ping, LI Wei，LI Yu-lin, ZHANG Li-hong, SHAO Ming-yu. Isolation of endothelial progenitor cells fromumbilical cord blood[J]. J4, 2004, 30(1): 150-152.

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