莪术油对中波紫外线辐射致豚鼠皮肤损伤的治疗作用

doi:吉林省科技厅资助课题（963003-1）

莪术油对中波紫外线辐射致豚鼠皮肤损伤的治疗作用

王志成¹, 杜翔¹ , 龚平生² , 郭伟¹, 李艳博¹, 康顺爱¹，李文兴¹，李校堃³, 龚守良^1＊

1. 吉林大学公共卫生学院卫生部放射生物学重点实验室，吉林长春 130021； 2. 吉林大学分子酶学工程教育部重点实验室，吉林长春 130023；3. 温州医学院生物与天然药物研究院, 浙江温州 325501

收稿日期:2005-10-20 修回日期:1900-01-01 出版日期:2006-11-28 发布日期:2006-11-28
通讯作者: 龚守良

Therapeutic effects of oleum curcumae wenchowensis on skin damage of guinea pigs irradiated by UVB

WANG Zhi-cheng¹, DU Xiang¹, GONG Ping-sheng²,GUO Wei¹, LI Yan-bo¹,KANG Shun-ai¹, LI Wen-xing¹ , LI Xiao-kun³, GONG Shou-liang^1＊

1. MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China; 2. Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun 130023, China; 3. Institute of Biology and Nature Drug, Wenzhou Medical College, Wenzhou 325501, China

Received:2005-10-20 Revised:1900-01-01 Online:2006-11-28 Published:2006-11-28
Contact: GONG Shou-liang

摘要/Abstract

摘要： 目的：研究莪术油对中波紫外线（ultraviolet B, UVB）所致豚鼠皮肤损伤的治疗作用及其机制。方法：UVB照射豚鼠皮肤，建立氧化损伤模型（照射30 d，累积28.38 J•cm^-2）。实验观察皮肤红斑形成、粗糙程度等外观变化及HE常规染色后光镜下表皮厚度和真皮中成纤维细胞数量的变化。酶法检测皮肤匀浆上清液中过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量及总抗氧化能力（T-AOC）。结果: ①光镜下可见UVB照射组和基质空白组照射侧表皮增厚，成纤维细胞数量减少，而未照射侧未见此病理变化；莪术油治疗组照射侧未见表皮增厚，而成纤维细胞增多；但未照射侧未见此病理变化。②与正常组比较，UVB照射组和基质空白组照射侧皮肤中MDA含量明显增多，CAT、GSH-Px和SOD活性及T-AOC降低；莪术油治疗组照射侧MDA减少，CAT、GSH-Px、SOD 活性及T-AOC增加；各组未照射侧无明显改变。结论: 莪术油具有对UVB皮肤氧化损伤的治疗作用，其作用机制可能与提高抗氧化酶含量及清除体内自由基有关。

关键词: 治疗应用, 紫外线, 抗氧化剂

Abstract: Objective To study the therapeutic effects of oleum curcumae wencho wensis on skin of guinea pigs damage irradiated by UVB and its mechanism. Methods The skin of guinea pigs was irradiated by UVB (28.38 J•cm^-2, 30 d) to establish the oxidative damage model. The skin erythema and the rough level were observed during the experiment; the appearance and thickness of epiderm and the number of fibroblast were observed under light microscope.The activities of GSH-Px, SOD, CAT and T-AOC and the content of MDA in the supernate of skin homogenate were detected with biochemical methods. Results The epiderm in UVB irradiated group and blank group thickened, the number of fibroblasts decreased;and the epiderm in therapeutic group didn't thicken, but the number of fibroblasts increased, but those in the unirradiated side of every group did not change. Compared with normal group, the contents of MDA in the supernate of skin homogenate in UVB irradiated group and blank group increased, but that in therapeutic group decreased. The activities of GSH-Px, SOD, CAT and T-AOC in UVB irradiated group and blank group decreased, but those in therapeutic group increased, and those in the unirradiated side of every group didn't change. Conclusion Oleum curcumae wenchowensis has the therapeutic effects on skin damage irradiated by UVB, which may be mediated by increasing the contents of antioxidases and eliminating the free radical.

Key words: therapeutic, ultraviolet , oxidant

中图分类号:

R285.5

王志成, 杜翔 , 龚平生 , 郭伟, 李艳博, 康顺爱，李文兴，李校堃, 龚守良. 莪术油对中波紫外线辐射致豚鼠皮肤损伤的治疗作用[J]. J4, 2006, 32(6): 1012-10121015.

WANG Zhi-cheng, DU Xiang, GONG Ping-sheng,GUO Wei, LI Yan-bo,KANG Shun-ai, LI Wen-xing , LI Xiao-kun, GONG Shou-liang. Therapeutic effects of oleum curcumae wenchowensis on skin damage of guinea pigs irradiated by UVB[J]. J4, 2006, 32(6): 1012-10121015.

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