关键词: 白细胞介素8, NF-κB , 二硫氨基甲酸吡啶

Abstract: Abstract:Objective To investigate the effect of pyrrolidine dithiocarbamate (PDTC) on the proliferation and expression of IL-8 of SKOV3 cells in acid environment,and the role of nuclear factor κB (NF -κB) in the process.Methods SKOV3 cell lines were divided into 5 groups: normal culture group,acid environment group，acid environment plus PDTC groups (25,50 and 100 mol•L^-1 PDTC).All the SKOV3 cells were cultivated for 12 h.The effect of PDTC on the proliferation of SKOV3 in the acid environment was determined by MTT,the content of IL-8 protein in the culture supernatant was detected by ELISA and the expression of NF-κB protein was measured by Western blotting.Results The IL-8 expression level in the acid environment was(1 384.211±42.320) ng•L^-1.there was significant difference compared with normal group（617.505 ng•L^-1±47.850 ng•L^-1)(P< 0.01).The IL-8 expression levels were decreased gradually with the enhancement of PDTC concentration (25,50, and 100 mol•L^-1),and the values were （1 239.053±14.490）,（1 113.578±29.140）and（1 014.457±55.49０） ng•L^-1，there were significant differences compared with acid environment group (P<0.05)．The deeper color protein strap of NF-κB in the SKOV3 cells was found in the acid environment and the color turned pale after SKOV3 cells were treated with PDTC. Conclusion PDTC could suppress the secretion of IL-8 in acid environment by supressing the activation of NF-κB.

Key words: interleukin 8, NF-kappaB, pyrrolidine dithiocarbamate