关键词: 角膜, 硅油类, 模型, 动物

Abstract: Abstract:Objective To investigate the effect of silicone oil on the corneal endothelium in experimental animal models,while the silicone oil in the anterior chamber of the experimental animals will be taken out so as to explore their corneal changes.Methods Forty healthy white rabbits were randomly divided into three groups : silicone oil in the anterior chamber group (SIG),silicone oil in and out of the anterior chamber group (SIOG) and control group(CG).The cornea of rabbit was investigated at different times by slit-lamp,corneal thickness instrument,endothelial cell count instrument,histology and electromicroscopy.The time of silicone oil in the anterior chamber without keratpathy and the pathological changes of cornea with taking out of the silicone oil were observed.Results The density of the corneal endothelium in SIOG and SIG decreased more obviously by endothelial cell count instrument from 8 weeks to 16 weeks compared with control group (P<0.01).The results of histology and electron microscopy showed that the core of cells shrank,many layers of corneal endothelium had been found.Cornea of SIOG was edematous by the slit-lamp.Compared with SIG,the corneal thickness in SIDG increased significantly (P<0.01);the fibers of corneal stroma in SIOG were loose and disorder.The corneal stroma thickness in SIOG increased lightly(P<0.01).The cornea in CG didn’t change obviously.Conclusion The damage of corneal endothelium of rabbit eyes will not recover if the silicone oil is in the anterior chamber beyond 8 weeks,more time come with more damage.

Key words: corneal, silicone oils, models, animal