关键词: 三硝基苯磺酸, 肠康饮, 疾病模型, 动物

Abstract: Objective To reproduce the inflammatory bowel disease（IBD） models i nduced by 2，4，6-trinitrobenzene sulfonic acid（TNBS），and to investigate the effect of Changkangyin（CKY） on it.Methods Total　 60 rats were divided into 6 groups by random：alcohol control group，model group，salicylazosulfapyridine （SASP）group，low，middle and high dosages CKY groups.IBD model was induced by TNBS.The changes of macroscopic morphous and pathohistology of colon were observ ed after intervened with drug for 21 d. Results The body weight in model group was obviously lower than that in alcohol control group （P<0.01）;the body weights in low，middle and high dosages CKY groups and SASP group were higher than that in model group（P<0.01）;the body weight in high dosage CKY group was higher than that in SASP group（P<0.01）.The scores of macroscopic morphous and pathohistology in model group were higher than those in alcohol control group obviously （P<0.01）; 　the scores in low，middle and high dosage CKY groups and SASP group were lower than those in model group obviously（P<0.01 or P<0.05）;there was no significant difference between high dosage CKY group and SASP group（P＞0.05）.Conclusion Colitis can be induced by TNBS in rats.The change of it is the same as human’s.It is a ideal model to study pathogenesis of IBD and investigate the effect of medicine.CKY has a batter therapeutic effect on IBD model.

Key words: trinitrobenzene sulfonic acid, Changkangyin, disease models, animal