溶血磷脂酸对豚鼠心肌细胞电压依赖性钾离子通道电流的作用

doi:吉林省自然科学基金资助课题

溶血磷脂酸对豚鼠心肌细胞电压依赖性钾离子通道电流的作用

李英骥，赵春燕^*，张文杰，李欣，卢静，李丽，钟国赣

吉林大学基础医学院生理学教研室，吉林长春 130021

收稿日期:2004-04-27 修回日期:1900-01-01 出版日期:2005-03-28 发布日期:2005-03-28
通讯作者: 赵春燕

Effects of lysophosphatidic acid on voltage dependent potassium current in myocytes of guinea pigs

LI Ying-ji,ZHAO Chun-yan^*,ZHANG Wen-jie,LI Xin,LU Jing,LI Li,ZHONG Guo-gan

Department of Physiology, School of Basic Medical Sciences,Jilin University,Changchun 130021, China

Received:2004-04-27 Revised:1900-01-01 Online:2005-03-28 Published:2005-03-28
Contact: ZHAO Chun-yan

摘要/Abstract

摘要： 目的：观察溶血磷脂酸(LPA)对豚鼠心肌细胞电压依赖性钾离子通道电流的作用。方法：用膜片钳实验技术记录胶原酶急性分离的豚鼠心室肌细胞钾通道电流。结果：共记录了33个心室肌细胞的电压依赖性外向K+通道电流发现1.0 μmol•L^-1和10.0 μmol•L^-1 LPA可明显降低K+通道电流的幅值。结论：LPA可能参与心肌梗塞时心律失常的发生。

关键词: 膜片钳技术, 心室肌细胞, 钾通道

Abstract: Objective To observe the effects of lysophosphatidic acid (LPA) on voltage dependent potassium current in myocytes of guinea pig. Methods The whole cell patch clamp technique was used in isolated ventricular cells of guinea pigs. Results Thirty-three voltage dependent potassium current in myocytes were recorded. LPA (1.0 and 10.0 μmol•L^-1) could significantly decrease the amplitude of the potassium current of ventricular myocytes. Conclusion LPA may participate in arrhythmia occurrence during myocardial infarction.

Key words: patch clamp technique, myocytes, potassium channels

中图分类号:

Q463

李英骥，赵春燕，张文杰，李欣，卢静，李丽，钟国赣. 溶血磷脂酸对豚鼠心肌细胞电压依赖性钾离子通道电流的作用[J]. J4, 2005, 31(2): 193-196.

LI Ying-ji,ZHAO Chun-yan,ZHANG Wen-jie,LI Xin,LU Jing,LI Li,ZHONG Guo-gan. Effects of lysophosphatidic acid on voltage dependent potassium current in myocytes of guinea pigs[J]. J4, 2005, 31(2): 193-196.

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