Abstract: Objective To inform the effects of Se and vitamin E on the anabolism of type Ⅰ collagen in cultured cardiac fibroblasts. Methods Cardiac fibroblasts were cultured using culture solution containing different concentrations of Se and vitamin E (VE), Se+VE for 48 h, then the contents of hydroxyproline and type Ⅰ collagen in cultured solution were detected. Results ①Compared with control and NE groups, the contents of hydroxyproline could be significantly decresed in all the three Se group (low 0.05 mg·L^-1, middle 0.1 mg·L^-1, high 0.2 mg·L^-1) (P<0.05), and this effect became apparent when the content of Se was 0.2 mg·L^-1 (56.13±1.31, P<0.05); ②Compared with control and NE groups, the contents of hydroxyproline were decreased significantly in all the three VE groups (1, 10, and 100 mg·L^-1), especially in 1 and 100 mg·L^-1 groups（P<0.05）; ③Both the contents of hydroxyproline and type Ⅰ collagen were decreased when Se (0.05 mg·L^-1) and VE (10 mg·L^-1) applied simultaneously. Conclusion The anabolism of type Ⅰ collagen in cultured cardiac fibroblasts can be inhibited by Se and VE.